等级
Molecular Biology
方案
>99% (SDS-PAGE)
表单
buffered aqueous solution
比活
>20,000 U/mg
浓度
5,000 U/mL
运输
dry ice
储存温度
−20°C
一般描述
Poly(A) Polymerase catalyzes the addition of AMP from ATP to the 3′ hydroxyl of RNA. The reaction requires Mg2+ and is template independent.
应用
Suitable for:
- Labeling of RNA with ATP or cordyceptin
- Poly(A) tailing of RNA for cloning or affinity purification
- Enhancing translation or RNA transferred into eukaryotic cells
特点和优势
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
外形
Supplied in 25 mM Tris-HCl, 500 mM NaCl, 1 mM MgCl2, 0.1 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 8.0 @ 25° C.
其他说明
1 unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-soluble material in 10 minutes at 37° C.
Source of protein: The gene encoding E. coli Poly(A) Polymerase expressed from a plasmid in E coli.
Supplied with:KEM0014B (Poly(A) Polymerase Reaction Buffer)KEM0070B (10 mM ATP solution)
Unit size: 1,000 U
储存分类代码
10 - Combustible liquids
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