grade
Molecular Biology
assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
625,000 U/mg
concentration
5,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
E. coli RNAse H (rnh) is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNAse H digestion produces ribonucleotide molecules with 5′-phosphate and 3′-hydroxyl termini. RNAse H is nearly inactive against single or double-stranded RNA molecules.
Application
Suitable for:
- Cleavage of RNA strand of a DNA:RNA hybrid
- Removing mRNA during second strand cDNA synthesis
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Physical form
Supplied in 20 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM DTT and 50% glycerol at pH @ 25° C.
Other Notes
1 unit is defined as the amount of enzyme that will hydrolyze 1 nmol of RNA from an 3H-labeled DNA:RNA hybrid molecule into acid-soluble material in 20 minutes at 37° C.
Source of protein: A recombinant E.coli strain carrying the RNAse H (rnh) gene.
Supplied with:KEM0015 B (10X RNAse H Buffer)
Unit size: 5,000 U
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
存储类别
10 - Combustible liquids
法规信息
新产品
此项目有
Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
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