等级
Molecular Biology
方案
>99% (SDS-PAGE)
表单
buffered aqueous solution
比活
300,000 U/mg
浓度
600,000 U/mL
运输
dry ice
储存温度
−20°C
一般描述
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
应用
Suitable for:
- Restriction cloning
- TA cloning
- Adapter ligation
- NGS library construction
- Other applications requiring high efficiency ligation
特点和优势
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
组分
Supplied with:KEM0046B (2X Rapid Ligation Buffer)KEM0049B (10X T4 DNA Ligase Buffer)
单位定义
1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 μL 1X DNA Ligase Buffer following a 30 minute incubation at 23° C.
外形
Supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 7.5 @ 25° C.
其他说明
Source of protein: A recombinant E. coli strain carrying the T4 DNA Ligase gene.
Unit size: 240,000 U
储存分类代码
10 - Combustible liquids
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