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About This Item

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等级

Molecular Biology

方案

>99% (SDS-PAGE)

表单

buffered aqueous solution

比活

300,000 U/mg

浓度

600,000 U/mL

运输

dry ice

储存温度

−20°C

一般描述

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

应用

Suitable for:
  • Restriction cloning
  • TA cloning
  • Adapter ligation
  • NGS library construction
  • Other applications requiring high efficiency ligation

特点和优势

  • Ultra-purification process for ultimate enzyme performance
  • Highest quality specifications for ultimate product consistency
  • Undetectable DNA and nuclease contamination

组分

Supplied with:KEM0046B (2X Rapid Ligation Buffer)KEM0049B (10X T4 DNA Ligase Buffer)

单位定义

1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 μL 1X DNA Ligase Buffer following a 30 minute incubation at 23° C.

外形

Supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 7.5 @ 25° C.

其他说明

Source of protein: A recombinant E. coli strain carrying the T4 DNA Ligase gene.
Unit size: 240,000 U

储存分类代码

10 - Combustible liquids


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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