T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

Suitable for:

• Restriction cloning
• TA cloning
• Adapter ligation
• NGS library construction
• Other applications requiring high efficiency ligation

Supplied with:KEM0046B (2X Rapid Ligation Buffer)KEM0049B (10X T4 DNA Ligase Buffer)

1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 μL 1X DNA Ligase Buffer following a 30 minute incubation at 23° C.

Supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 7.5 @ 25° C.

Source of protein: A recombinant E. coli strain carrying the T4 DNA Ligase gene.

Unit size: 240,000 U