grade
Molecular Biology
assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
16,800 U/mg
concentration
20,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded nucleic acids (RNA or DNA).
Application
Suitable for:
- Single stranded DNA ligation
- RNA ligation
- Labeling of 3′ termini of RNA
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Physical form
Supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 7.5 @ 25° C.
Other Notes
One unit is defined as the amount of enzyme required to ligate 50% of 0.4 μg of an equimolar mix of two single stranded 23 base RNA oligonucleotides (one 5′-phosphorylated) in 20 μL 1X T4 RNA Ligase Buffer following a 30 minute incubation at 37° C.
Source of protein: A recombinant E. coli strain carrying the T4 RNA Ligase gene from bacteriophage T4.
Supplied with:KEM0021B (10X T4 RNA Ligase Buffer)
Unit size: 10,000 U
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
存储类别
10 - Combustible liquids
法规信息
新产品
此项目有
Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
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