grade
Molecular Biology
assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
20,000-28,880 U/mg
concentration
10,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
E. coli DNA ligase catalyzes the phosphodiester bond formation between an adjacent 5′ phosphate and a 3′ hydroxyl of DNA ends, requiring NAD+ and Mg2+ as cofactors. Ligation of blunt ended DNA is extremely inefficient relative to cohesive DNA end ligation and nick sealing.
Application
Suitable for cDNA cloning by replacement synthesis.
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Physical form
Supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 7.5 @ 25° C.
Other Notes
1 unit is defined as the amount of E.coli DNA Ligase required to ligate 50% of 100 ng DNA fragments with cohesive termini in 30 minutes at 25° C.
Source of protein: The gene encoding E. coli DNA Ligase expressed from a plasmid in E. coli.
Supplied with:KEM0025B (10X E.coli DNA Ligase Reaction Buffer)
Unit size: 2,500 U
存储类别
10 - Combustible liquids
法规信息
新产品
此项目有
Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
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