Lectin from Limulus polyphemus (horseshoe crab)

Agglutinates Staphylococcus aureus cells, as well as human and horse erythrocytes.
Major affinity is for N-acetylated D-hexosamines, but is reported to have anti-galactan specificity.¹

Package size based on protein content

Lyophilized powder containing Tris succinate, NaCl and calcium acetate

Purified by affinity chromatography.

Agglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 7.4, containing 0.1 mM CaCl₂, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group B erythrocytes. Bacterial agglutination is determined from serial dilutions of a 1 mg/ml solution in 0.01 M Tris, 0.15 M NaCl, pH 7.4, 0.01M CaCl₂ with 1 mg/ml albumin and 0.92% TWEEN®.