type
Type I
form
ammonium sulfate suspension
specific activity
>40 units/mg protein
storage temp.
2-8°C
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General description
乳酸脱氢酶(LDH)的总分子量为140kDa,由4个亚基组成,称为M亚基(肌肉)和H亚基(心脏)。这些亚基可以以5种组合(M4、M3H1、M2H2、MH3和H4)中的任何一种进行混合。 骨骼肌含有LDH,其主要是M4,含有少量M3H和微量的H2H2。 H和M亚基的分子量非常相似,但氨基酸组成十分不同。兔肌肉LDH在pH5.0的乙酸盐-氯化物中可解离为二聚体物质(MW = ~70kDa),该解离是可逆的。Biochemistry, 13, 3527-3531 (1974). 氧化乙醛酸和乳酸。
等电点:8.4-8.6
最适pH值:7.5.
等电点:8.4-8.6
最适pH值:7.5.
Biochem/physiol Actions
其还可催化其他L-2-羟基单羧酸的氧化。
Physical form
Suspension in 3.2 M (NH4)2SO4 solution, pH 6.0
Analysis Note
Protein determined by biuret.
Other Notes
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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