L2395
D-Lactic Dehydrogenase from Leuconostoc mesenteroides
ammonium sulfate suspension, 1,000-3,000 units/mg protein (biuret)
别名:
Lactate, (R)-Lactate:NAD+ oxidoreductase, D-LDH
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
biological source
bacterial (Leuconostoc mesenteroides)
form
ammonium sulfate suspension
specific activity
1,000-3,000 units/mg protein (biuret)
storage temp.
2-8°C
Physical form
Suspension in 3.2 M (NH4)2SO4, 0.1 M potassium phosphate, pH 7.0
Other Notes
One unit will reduce 1.0 μmole of pyruvate to D-lactate per min at pH 7.0 at 25 °C.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
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Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
相关内容
QC Methods
Svetlana V Antonyuk et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 65(Pt 12), 1209-1213 (2010-01-08)
The crystal structure of D-lactate dehydrogenase from Aquifex aeolicus (aq_727) was determined to 2.12 A resolution in space group P2(1)2(1)2(1), with unit-cell parameters a = 90.94, b = 94.43, c = 188.85 A. The structure was solved by molecular replacement
Ting-Ting Xu et al.
Applied biochemistry and biotechnology, 160(2), 314-321 (2008-06-25)
The low-energy nitrogen ion beam implantation technique was used in the breeding of mutant D(-)-lactic-acid-producing strains. The wild strain Sporolactobacillus sp. DX12 was mutated by an N(+) ion beam with energy of 10keV and doses ranging from 0.4 x 10(15)
Takenori Satomura et al.
Journal of bioscience and bioengineering, 106(1), 16-21 (2008-08-12)
Dye-linked D-lactate dehydrogenase activity was found in the crude extract of a continental thermoacidophilic crenarchaeota, Sulfolobus tokodaii strain 7, and was purified 375-fold through four sequential chromatography steps. With a molecular mass of about 93 kDa, this enzyme was a