凝集素，来自Ricinus communis(蓖麻子)毒素 RCA₆₀–过氧化物酶标记

Ricinus communis agglutinin (RCA) occurs in two forms designated RCA₆₀ and RCA₁₂₀ according to their mol. wt. of approx. 60,000 and 120,000 respectively.¹ Neither RCA₆₀ nor RCA₁₂₀ is blood group specific. RCA₆₀, also referred to as RCA_II,² Ricin D³ or RCL III⁴ is extremely toxic, inhibits protein synthesis and has an affinity for N-acetyl-D-galactosamine. RCA₁₂₀, also referred to as RCA_I² or RCL (I + II)⁴ is an agglutinin and has an affinity for terminal β-D-galactosyl residues.¹
Conjugates are prepared from affinity purified toxin, RCA₆₀ or agglutinin RCA₁₂₀.

Package size based on protein content

含柠檬酸钠的冻干粉

Prepared from peroxidase type VI using two step glutaraldehyde method of Avrameas, S. and Ternyck, T., Immunochemistry, 8, 1175 (1971).
Repurified after conjugation by affinity chromatography.

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg protein per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25°C.

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20°C.