生物来源
Glycine max (soybean)
偶联物
peroxidase conjugate
方案
85.00-100.00% (Warburg-Christian)
表单
lyophilized powder
效能
<80 μg per mL agglutination activity
peroxidase activity
25-100 units/mg protein
组成
Protein, ~95% modified Warburg-Christian
技术
western blot: suitable
UniProt登记号
储存温度
−20°C
基因信息
soybean ... LE1(100818710)
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应用
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
生化/生理作用
SBA不是血型特异性的，但对N-乙酰-D-半乳糖胺具有亲和力。
外形
Contains sodium citrate
制备说明
由亲和纯化凝集素制备偶联物。
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
分析说明
凝集活性以 μg/ml 表示，并且在浓度为1 mg/ml、pH 6.8的磷酸缓冲盐水中进行连续稀释测定。该活性是在25℃温育1小时后，凝集2％人血型A红细胞悬浮液的最低浓度。
其他说明
One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0, at 20 °C.
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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