Anti-phospho-Lck (pTyr⁵⁰⁵) antibody produced in rabbit

The signalling in T cells is mediated by receptor and non-receptor protein tyrosine kinases (PTK). Among the family of non-receptors PTK, Lck is a lymphoid-specific cytosolic PTK essential for T-cell development and function. Lck is essential for selection and maturation of developing T cells in the thymus. It plays a significant role in T-cell receptors mediated signalling by constitutive association with CD4 and CD8 receptors. In addition, Lck undergoes phosphorylation and activation by association with CD2 molecule. Lck also plays a role in T-cell proliferation in response to IL-2 receptor pathway. The activity of Lck is known to be regulated by phosphorylation of two conserved tyrosine residues, tyrosine 505 (equivalent to tyrosine 529 in c-Src) and tyrosine 394 (equivalent to tyrosine 418 in c-Src). Phosphorylation at Tyr505 stabillizes Lck and renders it biologically inactive.
Anti-phospho Lck (pTyr⁵⁰⁵) specifically recognizes human Lck (pTyr⁵⁰⁵). Mouse (100% homology) and chicken (91.7% homology) are expected to react.

synthetic phosphopeptide derived from the region of Lck that is phosphorylated on tyrosine 505. Rat and mouse have 100% homology with human.

A working concentration of 0.1-1.0 μg/mL is recommended for detection of human Lck protein by immunoblotting.

Solution in phosphate buffer, pH 7.4, with 1 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

The antibody is preadsorbed to remove any reactivity towards a non-phosphorylated Lck.

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