L5006
亮氨酸氨基肽酶，微粒体 来源于猪肾脏
Type IV-S, ammonium sulfate suspension, 10-40 units/mg protein (Bradford)
别名:
亮氨肽酶, 亮酰胺氨肽酶, 氨基肽酶 M, 氨肽酶M, 氨肽酶N, 组织蛋白酶 III
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-942-5
MDL number:
Specific activity:
10-40 units/mg protein (Bradford)
type
Type IV-S
form
ammonium sulfate suspension
specific activity
10-40 units/mg protein (Bradford)
storage temp.
2-8°C
Quality Level
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General description
亮氨酸氨肽酶属于金属蛋白酶家族。
Application
猪肾来源微粒体亮氨酸氨肽酶用于从啮齿动物脑-C5 中分离脑啡肽酶（NEP），用于肽消化。
Biochem/physiol Actions
亮氨酸氨肽酶底物特异性广。能水解肽直至产生脯氨酸残基，不会对脯氨酸继续降解。
Physical form
3.5M (NH4)2SO4 溶液中的悬浮液，pH 7.7，含 10mM MgCl2
Other Notes
一个单位在37°C 下 pH 7.2 时每分钟将水解 1.0μ摩尔 L-亮氨酸-对硝基苯胺为 L -亮氨酸和对硝基苯胺（25°C 下可获得活性约为37°C 下的 40%）。以 L-亮氨酸对硝基苯胺为底物得到的活性是以 L-亮氨酸酰胺为底物得到的活性的 2-5 倍。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
The most potent organophosphorus inhibitors of leucine aminopeptidase. Structure-based design, chemistry, and activity
Grembecka J, et al.
Journal of medicinal chemistry, 46(13), 2641-2655 (2003)
Debittering of a Tryptic Digest of Bovine p-casein Using Porcine Kidney General Aminopeptidase and X-Prolydipeptidyl Aminopeptidase from Lactococcus lactis subsp. cremoris AM2
Barry CM, et al.
Journal of Food Science, 65(7), 1145-1150 (2000)
Sylvain Debieu et al.
Organic & biomolecular chemistry, 15(12), 2575-2584 (2017-03-08)
We report a reaction-based strategy for the fluorogenic detection of protease activity. Based on the "covalent-assembly" probe design principle recently put forward by the Yang group for detection of Sarin related threats (J. Am. Chem. Soc., 2014, 136, 6594-6597), we
Rossella Galati et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 58(7-8), 558-561 (2003-08-27)
The ability of synthetic protein fragments to survive the degradative action of aminopeptidases and serum proteolytic enzymes can be remarkably enhanced by slight modifications at their N-terminal alpha-amino group. This can be achieved by addition of beta-alanine or amino acids
Intestinal digestive resistance of immunodominant gliadin peptides
Hausch F, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 283(4), G996-G1003 (2002)
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
相关内容
QC Methods
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