form
lyophilized powder
potency
≤16 μg per mL agglutination activity (with erythrocytes), ≤10 μg per mL mitogenic activity
technique(s)
cell culture | mammalian: suitable
impurities
salt, free
solubility
PBS: 5 mg/mL
storage temp.
2-8°C
Biochem/physiol Actions
PHA由两种分子种类组成,即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素(PHA-E),和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素(PHA-L)。
PHA-E不具有血型特异性,但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前,PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。
PHA-E不具有血型特异性,但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前,PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。
Analysis Note
Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25°C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
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