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Merck
CN

L9132

来自 菜豆的凝集素 (红芸豆)

Phytohemagglutinin PHA-P, suitable for cell culture, lyophilized powder

别名:

PHA, 植物血球凝集素(菜豆)

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EC Number:
232-718-7
UNSPSC Code:
12352202
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form

lyophilized powder

potency

≤16 μg per mL agglutination activity (with erythrocytes), ≤10 μg per mL mitogenic activity

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

solubility

PBS: 5 mg/mL

storage temp.

2-8°C

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Biochem/physiol Actions

PHA由两种分子种类组成,即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素(PHA-E),和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素(PHA-L)。
PHA-E不具有血型特异性,但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前,PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。

Analysis Note

Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25°C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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Roxana Torres et al.
The Journal of experimental biology, 222(Pt 8) (2019-03-27)
Eggs are 'multivariate' in that they contain multiple maternally derived egg components (e.g. hormones, antibodies, mRNA, antioxidants) which are thought to influence offspring phenotype. However, most studies have focused on single egg components and on short-term effects. Here, we simultaneously
Tim H Scott-Taylor et al.
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, 119(3), 274-283 (2017-09-12)
Peripheral blood mononuclear cells (PBMCs) proliferate inconsistently, rendering current lymphoproliferation assays unreliable in diagnosis. To investigate the utility and nature of proliferation responses in allergy by comparison of the standard lymphoproliferation with a new dendritic cell (DC) stimulated assay. Monocyte-derived

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