偶联物
FITC conjugate
表单
lyophilized powder
组成
Protein, ~40% Lowry
标记范围
~2 mol FITC per mol protein
储存温度
−20°C
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一般描述
Lens culinaris lectin (LcL) is a carbohydrate-binding protein found in lentil seeds. It is an anti-nutritional compound and a heterotetramer containing two α-chains and two β-chains.
应用
Lectin from Lens culinaris (lentil) has been used:
- to stain oocytes during immunofluorescence staining for the detection of cortical granules
- in fluorescence quantification study
- lectin-binding assay
生化/生理作用
LcH 不具有血型特异性。对末端 α-D-甘露糖基和 α-D-葡萄糖基残基有亲和力。LcH 由两种异构体组成,LcH-A(分子量 49,000)和 LcH-B(分子量 49,000)。LcH-A 具有促有丝分裂活性。结合物由纯化的凝集素制备。
Lectins are implicated in the degeneration of red blood corpuscles. Therefore, lectin-containing foods must be properly cooked before consumption. It exhibits anti-viral activity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants by blocking the binding of angiotensin-converting enzyme 2 (ACE2) to the S-trimer protein of viruses. Lens culinaris lectin interacts with α-fetoprotein in the blood and serves as a potential marker for diagnosis of hepatocellular carcinoma. It also finds its application in affinity chromatography for the identification of glycoconjugates.
包装
Package size based on protein content.
外形
Lyophilized powder containing HEPES buffer salts and sodium chloride
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Shan Liu et al.
American journal of obstetrics and gynecology, 203(2), 151-151 (2010-06-29)
To investigate the role of cytoskeleton in several important dynamic events during in vitro maturation of human oocytes. Human germinal vesicle stage oocytes were divided randomly into control and study groups. After cultured for 24 hours, chromatin state and position
Wenbo Wang et al.
Emerging microbes & infections, 10(1), 1519-1529 (2021-07-20)
The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutated continuously and newly emerging variants escape from antibody-mediated neutralization raised great concern. S protein is heavily glycosylated and the glycosylation sites are relatively conserved, thus glycans on
Lily Anne Y Welty et al.
Acta histochemica, 107(6), 411-420 (2006-01-18)
For over a decade our laboratory has developed and used a novel histochemical assay using derivatized agarose beads to examine the surface properties of various cell types. Most recently, we have used this assay to examine lectin binding ligands on
Saponins and lectins of Indian chickpeas (Cicer arietinum) and lentils (Lens culinaris)
Srivastava RP and Vasishtha H
Indian Journal of Public Health Research & Development, 25(1), 44-47 (2012)
M Apparicio et al.
Reproduction in domestic animals = Zuchthygiene, 46(5), 896-903 (2011-03-01)
The aim of this study was to evaluate the effects of hCG, progesterone and oestradiol supplementation on nuclear and cytoplasmic maturation of canine oocytes cultured for 24, 48, 72 and 96 h. Oocytes obtained from 18 healthy bitches were divided
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