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LGC602101

BAC-Optimized Replicator v2.0 Electrocompetent Cells, (DUOs)

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pkg of 12 reactions (6x 50 μL), Cfu/µg ≥1 x 1010

别名:

BAC libraries cells

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About This Item

UNSPSC代码:
41106202

生物来源

Escherichia coli

制造商/商品名称

Lucigen 60210-1

包装

pkg of 12 reactions (6x 50 μL)

技术

microbiological culture: suitable

细胞转化

competent cell type: electrocompetent
transformation efficiency: 1×1010 cfu/μg

运输

dry ice

储存温度

-65 to -96°C

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一般描述

BAC-Optimised Replicator™ v2.0 Electrocompetent Cells are designed for BAC library construction or trasformation of large constructs. These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimised Replicator Cells are components of Biosearch Technologies Cloning Kits. Packed as DUOs (2 transformations per tube)

应用

  • Cloning
  • BAC Library construction
  • Cloning of large constructs

特点和优势

  • Highest available transformation efficiencies for library construction and screening
  • Ideal for BAC library construction or transformation of large inserts
  • Optimised for high yields of DNA
Transformation efficiency: ≥ 1 x 1010 (cfu/µg pUC or pKanR DNA))

Genotype BAC-Optimized Replicator v2.0: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galUgalK rpsL nupG (attL araC-PBAD-trfA250 bla attR) λ-. Note that Replicator v2.0 cells are AmpR (bla gene) (StrR)

Components:


  • BAC-Optimized Replicator v2.0 Electrocompetent Cells
  • Transformation Control pKanR
  • Arabinose Induction Solution
  • Recovery Media

法律信息

CopyRight is a registered trademark of Lucigen Corporation
Replicator is a trademark of Lucigen Corporation
pEZ is a trademark of Lucigen Corporation
pSMART is a registered trademark of Lucigen Corporation

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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