biological source
Escherichia coli
manufacturer/tradename
Lucigen 60514-1
packaging
pkg of 12 reactions (6 x 50 µL)
growth mode
growth or suspension
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: electrocompetent
transformation efficiency: 4 x 1010 cfu/μg
shipped in
dry ice
storage temp.
-65 to -96°C
General description
MC1061 F- is a non amber-suppressing strain that is commonly used for general cloning and Phage display because of its ability to reach very high transformation efficiencies. It is identical to Lucigen strain SS320, except that it lacks the F’ episome that is required for infection by filamentous phage such as M13. The strain can be used for plasmid propagation and phage display, but cannot be used for reinfection by filamentous phage.
Application
Phage Display Libraries, routine cloning, protein expression
Features and Benefits
- Perfect for antibody phage display library creation
- Lacks F’ episome, not suitable for M13 phage display
- Create larger libraries; speed recovery
- Supports cloning of methylated DNA, blue-white screening capable
Genotype: MC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1
Components:
MC1061 F- Electrocompetent Cells
pUC 19 Trasformation Control
Recovery Media
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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