Bst DNA Polymerase, Exo

Lucigen′s Bst DNA Polymerase, Exonuclease Minus, has higher strand displacement activity than that of other suppliers. The enzyme can be used in nucleic acid amplification methods such as isothermal amplification, whole genome amplification (WGA), and multiple displacement amplification (MDA).
Bst DNA polymerase has a high strand displacement activity. It is a recombinant form of the 67kDa Bacillus stearothermophilus DNA polymerase protein (large fragment). The enzyme has 5′-3′ polymerase activity and strand displacement activity, but it lacks 3′-5′ exonuclease activity. It also has reverse transcription activity. This enzyme has optimal activity at 65°C. It is suitable for sequencing DNA with high GC content and secondary structures. It is available in concentrations of 8,000 U/mL or 50,000 U/mL.

Bst DNA Polymerase, Exonuclease Minus can be used in-

• Isothermal amplification
• Whole genome amplification (WGA)
• Multiple displacement amplification (MDA)
• Next generation sequencing
• Strand displacement amplification
• DNA sequencing through high GC regions
• Rapid sequencing from nanogram amounts of DNA template

• Requires 0.1% Triton X-100 for long term storage
• Reaction temperatures above 70°C are not recommended
• Bst DNA polymerase cannot be used for thermal cycle sequencing

Heat Inactivation: 80ºC for 20 min.
Bst DNA Polymerase, Exonuclease Minus is available in two concentrations, 8,000 U/ml and 50,000 U/ml, in a storage buffer of 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, and 50% Glycerol. Also supplied is 10 X DNA Polymerase Buffer B, composed of 200 mM Tris-HCl pH 8.8, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, and 1.0 % Triton X-100.

Bst DNA Polymerase, Exonuclease Minus, is supplied with 10X DNA Polymerase Buffer B, composed of 200 mM Tris-HCl pH 8.8, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, and 1.0 % Triton X-100.