General description
phi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′ → 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.
Source: A recombinant Escherichia coli strain carrying the phi29 DNA polymerase gene from bacteriophage phi29.
Source: A recombinant Escherichia coli strain carrying the phi29 DNA polymerase gene from bacteriophage phi29.
Features and Benefits
Excellent strand displacement, fidelity and speed.
Other Notes
Supplied at 10,000 U/mL in 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol, pH 7.4 @ 25°C. Also supplied is 10X phi29 DNA Polymerase Buffer composed of 500 mM Tris-HCl, 100 mM (NH4)2SO4, 40 mM Dithiothreitol, 100 mM MgCl2, pH 7.5 @ 25°C. 10,000 U is supplied as 5 × 2,000 U.
Legal Information
NxGen is a registered trademark of Lucigen Corporation
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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