M3567
Anti-Myosin Iβ (Nuclear) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
别名:
Anti-Myh 2, Anti-Myo1c, Anti-Myr 2
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IF, WB
Citations:
14
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~120 kDa
species reactivity
human, mouse, canine, rat
technique(s)
indirect immunofluorescence: 1:50 using mouse NIH3T3 cells, microarray: suitable, western blot: 1:1,000 using extract of human HeLa cells nuclei and dog MDCK cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MYO1C(4641)
mouse ... Myo1c(17913)
rat ... Myo1c(65261)
General description
Myosins belong to a superfamily of actin-based motor proteins comprising to date at least 15 classes. Myosin I (also designated myosin-1c, myo1c, M1C, myr 2 and myh 2) is a widely distributed single-headed myosin composed of an N-terminal motor domain, a calmodulin-light chain binding neck region, and a short C-terminal domain. In mammalian cells, myosin I is usually found in the cytoplasm and is especially enriched in perinuclear regions and dynamic cell margins. Myosin Iβ is found in a variety of cells such as kidney tubular cells and inner-ear hair cells.
Immunogen
synthetic peptide corresponding to amino acid residues 1-16 of the heavy chain of mouse myosin Iβ (nuclear form). This sequence is missing from the other myosin I isoforms as well as from other currently known myosins.
Application
Anti-Myosin Iβ (Nuclear) antibody produced in rabbit has been used in:
- western blot
- confocal immunofluorescence
- immunogold labeling
- immunofluorescence
- co-immunoprecipitation experiments
- immunostaining
Biochem/physiol Actions
Myosin I is involved in cell motility, microvilli anchorage, vesicular and organelle transport and signal transduction. Myosin I associate with lipid membranes through its tail domain while its motor domain interacts with actin filaments. Myosin Iβ (nuclear) co-localizes with RNA polymerases I and II and possibly acts as a molecular motor to power transcription.
Recognizes the heavy chain of the nuclear form of human myosin Iβ. Additional bands may be detected in some preparations.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Motor domain-dependent localization of myo1b (myr-1)
Tang N, et al.
Current Biology, 11(14), 1131-1135 (2001)
Nuclear myosin I regulates cell membrane tension
Venit T, et al.
Scientific Reports, 30864-30864 (2016)
Subnuclear compartmentalization and function of actin and nuclear Myosin I in plants
Cruz JR, et al.
Chromosoma, 118(2), 193-207 (2009)
Force Generation by Membrane-Associated Myosin-I
Pyrpassopoulos S, et al.
Scientific reports (2016)
Specific nuclear localizing sequence directs two myosin isoforms to the cell nucleus in calmodulin-sensitive manner
Dzijak R, et al.
PLoS ONE, 7(1), e30529-e30529 (2012)
相关内容
Datasheet
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