Monoclonal Antibodies: A Practical Approach

Considers the preparation, testing, derivation and applications of monoclonal antibodies. New immunological techniques incorporating tried and tested methodologies are described. Both the standard somatic hybridization technique and recombinant techniques are described along with both small and large scale production, purification and labeling. Protocols are given for the use of monoclonal antibodies in rheumatoid arthritis, tissue typing, detecting DNA modified during chemotherapy, and in the clinical analysis of transplantation samples for malignancy