一般描述
Murashige & Skoog培养基广泛用于植物组织生长培养基。 M&S基础培养基含有大量营养素，包括高含量的硝酸盐和有机添加剂，如琼脂、糖、维生素和生长调节剂。 经常添加到M&S中的重要生长调节剂包括IAA（生长素/形态发生素）和激动素（细胞分裂素/细胞分裂促进剂）。
应用
Murashige & Skoog 基础盐混合物（MS）已用于维持转化的烟草细胞的生长和再生。它也可用作拟南芥 (Col-0)种子发芽的生长培养基。
制备说明
Murashige & Skoog培养基可以由粉末复溶，或者将作为M&S完全培养基主要成分（大量营养素混合物和维生素混合物）的产品混合后复溶。 Murashige & Skoog盐混合物（M5524）包含原始经典配方中的大量营养素和微量营养素。它可以与M&S维生素或Gamborg维生素结合，辅以蔗糖、琼脂、生长素（IAA）和细胞分裂素（激动素），形成植物组织生长完全培养基。
配制成每升培养基含 4.3 克粉末。
其他说明
储存
粉末培养基极易吸湿，必须防止空气中的水分对其潮解。单个包装的全部粉末应尽可能在打开后立即使用。
将干燥培养基保存在0-5°C的干燥器中。
粉末培养基变质可以通过以下方式识别：1）颜色变化；2）粉末中的颗粒化、结块或颗粒物；3）不溶性；4）pH变化；或5）正确使用时无法促进生长。
培养基制备
粉末培养基极易吸湿，必须防止空气中的水分对其潮解。单个包装的全部粉末应尽可能在打开后立即使用。
将干燥培养基保存在0-5°C的干燥器中。
粉末培养基变质可以通过以下方式识别：1）颜色变化；2）粉末中的颗粒化、结块或颗粒物；3）不溶性；4）pH变化；或5）正确使用时无法促进生长。
培养基制备
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Ox. Sol. 3
储存分类代码
5.1B - Oxidizing hazardous materials
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
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