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Merck
CN

M5813

Sigma-Aldrich

仿橙2号配体亲和吸附剂

suspension

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UNSPSC代码:
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表单

suspension

运输

wet ice

储存温度

2-8°C

应用

Ligand affinity adsorbents are used in protein chromatography, affinity chromatography and dye resins. Ligand affinity adsorbents have been used to describe a simple protocol for the purification of Taq Polymerase from E. coli lysates as well as for the purification of other DNA polymerases.

象形图

Flame

警示用语:

Warning

危险声明

危险分类

Flam. Liq. 3

储存分类代码

3 - Flammable liquids

WGK

WGK 1

闪点(°F)

95.0 °F - closed cup

闪点(°C)

35 °C - closed cup

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

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分析证书(COA)

Lot/Batch Number

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Huma Khan et al.
The Journal of biological chemistry, 279(29), 30563-30572 (2004-05-07)
The structure of pentaerythritol tetranitrate (PETN) reductase in complex with the nitroaromatic substrate picric acid determined previously at 1.55 A resolution indicated additional electron density between the indole ring of residue Trp-102 and the nitro group at C-6 of picrate.
Biological activity and biosynthesis of pentacyclic oxylipins: The linoleic acid pathway.
Gundlach, H., and Zenk, M.H.
Phytochemistry, 47(4), 527-537 (1998)
Huma Khan et al.
The FEBS journal, 272(18), 4660-4671 (2005-09-15)
The roles of His181, His184 and Tyr186 in PETN reductase have been examined by mutagenesis, spectroscopic and stopped-flow kinetics, and by determination of crystallographic structures for the Y186F PETN reductase and reduced wild-type enzyme-progesterone complex. Residues His181 and His184 are
Huma Khan et al.
The Journal of biological chemistry, 277(24), 21906-21912 (2002-03-30)
The reaction of pentaerythritol tetranitrate reductase with reducing and oxidizing substrates has been studied by stopped-flow spectrophotometry, redox potentiometry, and X-ray crystallography. We show in the reductive half-reaction of pentaerythritol tetranitrate (PETN) reductase that NADPH binds to form an enzyme-NADPH
Crystal structure of pentaerythritol tetranitrate reductase: "flipped" binding geometries for steroid substrates in different redox states of the enzyme.
Barna, T.M., et al.
Journal of Molecular Biology, 310(2), 443-447 (2001)

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