生物来源
mouse
重组
expressed in E. coli
方案
≥97% (SDS-PAGE and N-terminal analysis)
表单
lyophilized powder
效能
40-100 ng/mL ED50/EC50
分子量
7.8 kDa
包装
pkg of 10 μg
储存条件
avoid repeated freeze/thaw cycles
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
UniProt登记号
储存温度
−20°C
基因信息
mouse ... Ccl4(20303)
一般描述
MIP-1α and MIP-1β (macrophage inflammatory protein) were originally co-purified from LPS stimulated mouse macrophages. Recombinant, mouse MIP-1β (rmMIP-1β) consists of 69 amino acids. MIP- 1β belongs to the chemokine β subfamily which is characterized by a C-C configuration at the first two cysteines. MIP-1β has endogenous pyrogenic activity when it is injected intravenously into rabbits. Although other cytokines, such as IL-1alpha, IL-1β and TNF have endogenous pyrogenic activity, the pyrogenic effects of these cytokines can be inhibited by cyclooxgenase blockers, while the pyrogenicity of MIP- 1β is unaffected by these agents. MIP-1β can synergize with the hematopoietic growth factors granulocyte macrophage CSF (GM-CSF) or macrophage CSF (M-CSF) to enhance colony formation.
生化/生理作用
MIP-1β has endogenous pyrogenic activity when it is injected intravenously into rabbits. MIP-1 can synergize with the hematopoietic growth factors granulocytemacrophage CSF (GM-CSF) or macrophage CSF (M-CSF) to enhance colony formation.
外形
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin
分析说明
The biological activity of MIP-1β was tested in culture by measuring its ability to inhibit hematopoietic stem cell proliferation in an in vitro colony assay.
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