M7433
Anti-phospho-MAP Kinase Kinase 4 (MKK4, SEK, JNKK1) (pThr223) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous glycerol solution
种属反应性
human, mouse, rat
技术
western blot (chemiluminescent): 1:2,000 using sodium chloride and vanadate-treated 293 cells
UniProt登记号
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pThr223)
基因信息
human ... MAP2K4(6416)
mouse ... Map2k4(26398)
一般描述
SAPK/ERK-1 kinase (SEK1) also called MKK4 or Jun kinase kinase (JNKK) activates the stress activated protein kinase (SAPK). There are other mechanisms by which SAPK may be activated but SEK1 being the immediate upstream activator defines the ensuing protein kinase cascade by the c-Jun transcription factor. Activation of SEK1 occurs through phosphorylation of serine and threonine residues at position 219 and 223 respectively by MEKK. High levels of JNK/SAPK activity are reported to induce apoptosis by the activation of c-Jun. Anti-phospho-MAP Kinase Kinase 4 (MKK4, SEK1, JNKK1 (phosphothreonine 223) detects SEK1/MKK4 protein when activated by phosphorylation at Thr223.
免疫原
synthetic phospho-Thr223 peptide corresponding to residues around Thr223 of human SEK1.
应用
Anti-phospho-MAP Kinase Kinase 4 may be used for immunoblotting at a working dilution of 1:2,000 using sodium chloride and vanadate-treated 293 cells.
生化/生理作用
Does not react with non-phosphorylated MKK4/SEK1 or phosphorylated MKK3, MEK-1 or MEK-2.
外形
Solution in 10 mM HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/mL bovine serum albumin and 50% glycerol.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
此项目有
I Sánchez et al.
Nature, 372(6508), 794-798 (1994-12-22)
The stress-activated protein kinases (SAPKs), which are distantly related to the MAP kinases, are the dominant c-Jun amino-terminal protein kinases activated in response to a variety of cellular stresses, including treatment with tumour-necrosis factor-alpha and interleukin-beta (refs 1, 2). SAPK
Brian S Johnson et al.
Nature medicine, 23(10), 1203-1214 (2017-09-19)
Mutations in MECP2 cause Rett syndrome (RTT), an X-linked neurological disorder characterized by regressive loss of neurodevelopmental milestones and acquired psychomotor deficits. However, the cellular heterogeneity of the brain impedes an understanding of how MECP2 mutations contribute to RTT. Here
J M Kyriakis et al.
Nature, 369(6476), 156-160 (1994-05-12)
The mitogen-activated protein (MAP) kinases Erk-1 and Erk-2 are proline-directed kinases that are themselves activated through concomitant phosphorylation of tyrosine and threonine residues. The kinase p54 (M(r) 54,000), which was first isolated from cycloheximide-treated rats, is proline-directed like Erks-1/2, and
R J Davis
Trends in biochemical sciences, 19(11), 470-473 (1994-11-01)
Mitogen-activated protein kinases (MAPKs) are activated by dual phosphorylation on threonine and tyrosine in response to a wide array of extracellular stimuli. In the yeast Saccharomyces cerevisiae, a series of extracellular stimuli. In the yeast Saccharomyces cerevisiae, a series of
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