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Merck
CN

M8280

Sigma-Aldrich

Murashige & Skoog 改良基础盐混合物

powder, suitable for plant cell culture

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UNSPSC代码:
12352207
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表单

powder

氮含量分析

40 mM total

技术

cell culture | plant: suitable

应用

agriculture

储存温度

2-8°C

应用

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

生化/生理作用

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M8280) contain the macronutrients and micronutrients of the original classic formulation with sodium nitrate substituted for ammonium nitrate. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

制备说明

通过用硝酸钠替代硝酸铵改良。

其他说明

无 NH4
如 Murashige 和 Skoog (1962) 所述含常量营养素和微量营养素。

法规信息

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分析证书(COA)

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Gabriele B Monshausen et al.
The Plant cell, 21(8), 2341-2356 (2009-08-06)
Mechanical stimulation of plants triggers a cytoplasmic Ca(2+) increase that is thought to link the touch stimulus to appropriate growth responses. We found that in roots of Arabidopsis thaliana, external and endogenously generated mechanical forces consistently trigger rapid and transient

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