Anti-phospho-c-Myc (pThr⁵⁸/pSer⁶²) antibody produced in rabbit

The transcription factor c-Myc is a short-lived nuclear phosphoprotein involved in cell proliferation, differentiation, and cancer. Expression of c-Myc is strictly controlled by mitogens and is required for cell cycle entry. c-Myc binds to DNA and activates transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro at Ser⁶² by MAP Kinase, and in vivo at both Thr⁵⁸ and Ser⁶² although the pathways and kinases acting in vivo have not been identified. Mutation of Thr⁵⁸ and Ser⁶² to Ala inhibits the ability of c-Myc to stimulate transcription. c-Myc, a ~45 kDa protein that migrates with an apparent molecular weight of ~64-67kDa in reducing SDS-PAGE, appears to regulate multiple aspects of growth control; c-Myc promotes cell proliferation in the presence of Cdk inhibitors and induces apoptosis upon overexpression in serum deprived cells.

synthetic peptide corresponding to residues around phospho-Thr⁵⁸ and phospho-Ser⁶² of human c-Myc.

Does not react with non-phosphorylated c-Myc.

In phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Purified by protein A and peptide affinity chromatography.

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.

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