Mrp2 from rat

MRP2 (ABCC2) is an organic anion transporter found in liver, kidney, and gut epithelium apical membranes. The transport of glucuronate conjugates plays a role in the detoxification of endogenous and xenobiotic substances, and may cause multidrug resistance (MDR) in tumor cells. The rat Mrp2 transporter shows 72.3% sequence identity and 85.6% sequence similarity with human MRP2. Both transporters are expressed on the canalicular membrane of the liver and are known to be responsible for the transport of some organic molecules and their conjugates to the bile.

Detection of ATPase activity of the Mrp2 protein is a measure of transporter activity. The assay is performed using purified membrane vesicles from Sf9 (Spodoptera frugiperda) cells, expressing high levels of Mrp2 protein. ABC transporters pump substrates out of the cell by using hydrolysis of ATP as an energy source. ATP hydrolysis yields inorganic phosphate (P_i), which can be detected by a simple colorimetric reaction. The amount of P_i liberated is proportional to the activity of the transporter.

Supplied as isolated Sf9 cell membranes containing rat Mpr2 suspended in TMEP solution.

Membrane preparations containing ABC transporters show a baseline ATPase activity that varies for different transporters. Transported substrates increase this baseline ATPase activity, while inhibitors or slowly transported compounds inhibit the baseline ATPase activity and/or the ATPase activity measured in the presence of a stimulating agent. Both activation and inhibition studies can be performed.

Distributed for SOLVO Biotechnology, Inc.