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Merck
CN

MAK054

α-酮戊二酸检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

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关于此项目

NACRES:
NA.84
UNSPSC Code:
12161503
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usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric, fluorometric

relevant disease(s)

cancer

storage temp.

−20°C

General description

α-酮戊二酸(α-KG)是三羧酸(TCA)循环中的关键中间体,是由异柠檬酸脱氢酶对异柠檬酸的氧化脱脂作用形成的。 通过谷氨酸脱氢酶催化谷氨酸合成 α-KG 是TCA 循环的厌氧反应的关键步骤。α-KG 是重要的氮转运蛋白。这种 α-酮戊二酸测定试剂盒提供了一种简单、灵敏、快速的方法,用于量化各种样品中的 α-KG。

Application

α-酮戊二酸测定试剂盒已用于测定 α-酮戊二酸的活性。
适用于测定细胞和组织裂解物等多种生物样本中的a-酮戊二酸

Biochem/physiol Actions

通过偶联酶测定法测定 α-KG浓度,其产生与存在的 α-KG成比例的比色(570nm)/荧光(λex = 535/λem = 587 nm)产物。该试剂盒的典型检测灵敏度为 0.01 至 10 nmoles。

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

法规信息

常规特殊物品
低风险生物材料
此项目有

历史批次信息供参考:

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Phoebe J Benson et al.
Frontiers in microbiology, 7, 475-475 (2016-04-20)
Interest in the production of carbon commodities from photosynthetically fixed CO2 has focused attention on cyanobacteria as a target for metabolic engineering and pathway investigation. We investigated the redirection of carbon flux in the model cyanobacterial species, Synechococcus elongatus PCC
Gunars Osis et al.
Physiological reports, 4(8) (2016-04-28)
The bicarbonate transporter, NBCe1 (SLC4A4), is necessary for at least two components of the proximal tubule contribution to acid-base homeostasis, filtered bicarbonate reabsorption, and ammonia metabolism. This study's purpose was to determine NBCe1's role in a third component of acid-base
Glutamine transporters are targets of multiple oncogenic signaling pathways in prostate cancer
White M A, et al.
Molecular Cancer Research, 480-480 (2017)
Shaohui Chen et al.
Stem cell research, 49, 102097-102097 (2020-12-04)
The developmental plasticity of embryonic stem cells (ESCs) is mainly controlled by well-characterized transcription factors, but additional factors, especially those related to metabolism that modulate this intrinsic program remain elusive. Here, using whole transcriptome analysis, we identified branched-chain amino acid
Jianjian Zhao et al.
Bioscience reports, 40(3) (2020-03-04)
The activation of hepatic stellate cells (HSCs) plays a central role in liver fibrosis. α-ketoglutarate is a natural metabolite and previous studies have shown that increase in intracellular α-ketoglutarate can inhibit HSC activation. The aim of the present study is

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