usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric, fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
General description
α-酮戊二酸（α-KG）是三羧酸（TCA）循环中的关键中间体，是由异柠檬酸脱氢酶对异柠檬酸的氧化脱脂作用形成的。 通过谷氨酸脱氢酶催化谷氨酸合成 α-KG 是TCA 循环的厌氧反应的关键步骤。α-KG 是重要的氮转运蛋白。这种 α-酮戊二酸测定试剂盒提供了一种简单、灵敏、快速的方法，用于量化各种样品中的 α-KG。
Application
α-酮戊二酸测定试剂盒已用于测定 α-酮戊二酸的活性。
适用于测定细胞和组织裂解物等多种生物样本中的a-酮戊二酸
Biochem/physiol Actions
通过偶联酶测定法测定 α-KG浓度，其产生与存在的 α-KG成比例的比色（570nm）/荧光（λex = 535/λem = 587 nm）产物。该试剂盒的典型检测灵敏度为 0.01 至 10 nmoles。
signalword
Danger
存储类别
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
wgk
WGK 3
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
法规信息
常规特殊物品
低风险生物材料
此项目有
Phoebe J Benson et al.
Frontiers in microbiology, 7, 475-475 (2016-04-20)
Interest in the production of carbon commodities from photosynthetically fixed CO2 has focused attention on cyanobacteria as a target for metabolic engineering and pathway investigation. We investigated the redirection of carbon flux in the model cyanobacterial species, Synechococcus elongatus PCC
Gunars Osis et al.
Physiological reports, 4(8) (2016-04-28)
The bicarbonate transporter, NBCe1 (SLC4A4), is necessary for at least two components of the proximal tubule contribution to acid-base homeostasis, filtered bicarbonate reabsorption, and ammonia metabolism. This study's purpose was to determine NBCe1's role in a third component of acid-base
Shota Kawakami et al.
Oncology letters, 20(6), 351-351 (2020-10-31)
Isocitrate dehydrogenase 1 (IDH1) mutations are common in gliomas, acute myeloid leukemia, and chondrosarcoma. The mutation 'hotspot' is a single arginine residue, R132. The R132H mutant of IDH1 produces the 2-hydroxyglutarate (2-HG) carcinogen from α-ketoglutarate (α-KG). The reduction of α-KG
Glutamine transporters are targets of multiple oncogenic signaling pathways in prostate cancer
White M A, et al.
Molecular Cancer Research, 480-480 (2017)
Shaohui Chen et al.
Stem cell research, 49, 102097-102097 (2020-12-04)
The developmental plasticity of embryonic stem cells (ESCs) is mainly controlled by well-characterized transcription factors, but additional factors, especially those related to metabolism that modulate this intrinsic program remain elusive. Here, using whole transcriptome analysis, we identified branched-chain amino acid
商品
Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.
本文介绍了增殖活性细胞为何需要碳源和氮源合成大分子。尽管大部分肿瘤细胞利用有氧糖酵解途径并分流线粒体氧化磷酸化代谢物，但许多肿瘤细胞表现出线粒体活性增加。
实验方案
We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持