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安全信息

MAK070

Sigma-Aldrich

草酰乙酸检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

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NACRES:
NA.84

用途

sufficient for 100 colorimetric or fluorometric tests

检测方法

colorimetric
fluorometric

相关疾病

cancer

储存温度

−20°C

一般描述

草酰乙酸 (OAA) 是三羧酸循环和糖异生的中间产物。在柠檬酸盐之前，通过柠檬酸盐合成酶介导的乙酰辅酶 a 和 OAA 缩合形成柠檬酸盐。OAA 也可由天冬氨酸脱酰胺或 CO 2 与丙酮酸或 PEP 缩合形成。由于哺乳动物不具备从乙酰辅酶 a 形成三羧酸循环中间产物的酶促机制，因此 OAA 是通过丙酮酸和丙酮酸羧激酶的回补进入点之一。条目草酰乙酸测定试剂盒提供了一种简单、灵敏和快速的方法，可定量测定多种样品中的 OAA。

应用

草酰乙酸测定试剂盒用于测定样品中草酰乙酸的浓度。

适用性

适用于动物组织、细胞培养（贴壁细胞或悬浮细胞）、血清和唾液。

原理

在本试剂盒中，通过偶联酶试验测定草酰乙酸盐浓度，得到比色 (570 nm)/荧光 (lex = 535/lem = 587 nm) 产物，与存在的草酰乙酸成比例。该试剂盒的典型检测范围为 2-10 nmole（比色法）和 0.2-1 nmole（荧光法）。

替代产品

产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H317 - H319 - H334

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 3

法规信息

常规特殊物品

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示例

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  1. Why is deproteinizing necessary for this assay?

    We suggest deproteinizing samples using a PCA/KOH protocol (BioVision, Cat. #K808-200) or passing the samples through 10 kDa molecular weight cut off spin columns (BioVision, Cat # 1997-25). This will get rid of much of the protein in the samples and can remove the enzymes which can be converting OAA to pyruvate already.

  2. Can this kit be used with samples like bacteria, plants, drosophila, yeast etc?

    We have optimized the kit with mammalian samples. However, theoretically these kits should work with samples from multiple species/sources. OAA is conserved through evolution. Since the optimal conditions depend on the sample type, the protocol has to be be adapted to fit the samples for efficient results. Please refer to this kit's citations to see what kind of samples have been used with this kit other than mammalian samples.

  3. Can frozen samples be used with this assay?

    Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.

  4. Is it possible to use a different wavelength than recommended for the final analysis?

    It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.

  5. What is the exact volume of sample required for this assay?

    There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. It is recommended to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.

  6. Are trial sizes of this kit available?

    Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted.

  7. Can individual components of this kit be purchased separately?

    Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.

Ke Tang et al.
Oncogene, 38(44), 6970-6984 (2019-08-15)
Clinical applications of antiangiogenic agents profoundly affect tumor cell behaviors via the resultant hypoxia. To date, how the hypoxia regulates tumor cells remains unclear. Here, we show that hypoxia promotes the growth of human breast tumorigenic cells that repopulate tumors
Zheng Li et al.
Stem cells (Dayton, Ohio), 37(12), 1542-1555 (2019-10-02)
Mitochondrial phosphoenolpyruvate carboxykinase (PCK2) is a rate-limiting enzyme that plays critical roles in multiple physiological processes. The decompensation of PCK2 leads to various energy metabolic disorders. However, little is known regarding the effects of PCK2 on osteogenesis by human mesenchymal
Huabo Wang et al.
The Journal of biological chemistry, 294(14), 5466-5486 (2019-02-14)
Eukaryotic cell metabolism consists of processes that generate available energy, such as glycolysis, the tricarboxylic acid (TCA) cycle, and oxidative phosphorylation (Oxphos), and those that consume it, including macromolecular synthesis, the maintenance of ionic gradients, and cellular detoxification. By converting
The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing.
Bartok O, Teesalu M, Ashwall-Fluss R, et al.
The Embo Journal, 34(11), 1538-1553 (2015)
The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing.
Bartok O, et al.
The Embo Journal, 34(11), 1538-1553 (2015)
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