usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric, fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
General description
草酰乙酸 (OAA) 是三羧酸循环和糖异生的中间产物。在柠檬酸盐之前，通过柠檬酸盐合成酶介导的乙酰辅酶 a 和 OAA 缩合形成柠檬酸盐。OAA 也可由天冬氨酸脱酰胺或 CO 2 与丙酮酸或 PEP 缩合形成。由于哺乳动物不具备从乙酰辅酶 a 形成三羧酸循环中间产物的酶促机制，因此 OAA 是通过丙酮酸和丙酮酸羧激酶的回补进入点之一。条目草酰乙酸测定试剂盒提供了一种简单、灵敏和快速的方法，可定量测定多种样品中的 OAA。
Application
草酰乙酸测定试剂盒用于测定样品中草酰乙酸的浓度。
适用于动物组织、细胞培养（贴壁细胞或悬浮细胞）、血清和唾液。
Biochem/physiol Actions
在本试剂盒中，通过偶联酶试验测定草酰乙酸盐浓度，得到比色 (570 nm)/荧光 (lex = 535/lem = 587 nm) 产物，与存在的草酰乙酸成比例。该试剂盒的典型检测范围为 2-10 nmole（比色法）和 0.2-1 nmole（荧光法）。
signalword
Danger
存储类别
10 - Combustible liquids
wgk
WGK 3
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1
法规信息
常规特殊物品
此项目有
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The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing.
Bartok O, Teesalu M, Ashwall-Fluss R, et al.
The Embo Journal, 34(11), 1538-1553 (2015)
商品
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本文介绍了增殖活性细胞为何需要碳源和氮源合成大分子。尽管大部分肿瘤细胞利用有氧糖酵解途径并分流线粒体氧化磷酸化代谢物，但许多肿瘤细胞表现出线粒体活性增加。
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