产品名称
葡萄糖摄取荧光测定试剂盒, sufficient for 100 fluorometric tests
usage
sufficient for 100 fluorometric tests
detection method
fluorometric
relevant disease(s)
endocrinological disorders, diabetes; cancer
storage temp.
−20°C
Application
葡萄糖摄取检测试剂盒（荧光法）已可用于测量单核细胞样髓源抑制细胞（M-MDSC）的葡萄糖摄取量。
适用于检测在96孔微量滴定板中培养的贴壁细胞或悬浮细胞的葡萄糖摄取。
Biochem/physiol Actions
葡萄糖摄取检测试剂盒（荧光法）可简单、直接地测定多种细胞的葡萄糖摄取能力。该试剂盒使用葡萄糖类似物2-脱氧葡萄糖（2-DG）测量葡萄糖摄取，2-DG在测定中被细胞摄取并被己糖激酶磷酸化为2-DG6P。2-DG6P无法进入后续代谢步骤，因而会在细胞中聚集，直接成比例地反映细胞的葡萄糖摄取水平。在该测定中,2-DG的摄取水平通过偶联酶促反应方法测定,其中，2-DG6P在此过程中被氧化生成NADPH，随后NADPH与探针反应，生成的荧光（λex = 535/λem = 587 nm）产物与细胞的2-DG摄取量成比例。
General description
现在我们可提供全新的葡萄糖摄取检测试剂盒MAK505！葡萄糖是大多数细胞的主要能量来源。葡萄糖的跨细胞膜运输是葡萄糖代谢中的第一个限速步骤。GLUT家族的转运蛋白可促进葡萄糖摄取，而这些蛋白的表达和活性受到多种机制的调控。许多癌细胞的葡萄糖摄取增加，表现出高速率的有氧糖酵解。胰岛素抵抗细胞表现出更少的胰岛素刺激后葡萄糖摄取。
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
存储类别
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
法规信息
常规特殊物品
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