用途
sufficient for 200 fluorometric tests
检测方法
fluorometric
相关疾病
cancer
储存温度
−20°C
一般描述
谷氨酸氧化酶是一种氧化还原酶，能够催化L-谷氨酸氧化脱氨基生成酮戊二酸、氨和过氧化氢。
谷氨酸氧化酶检测试剂盒可简单、直接地测定溶液和细胞提取物等多种样品的谷氨酸氧化酶活性。该试剂盒通过酶偶联法测定谷氨酸氧化酶的活性，谷氨酸的氧化将产生与谷氨酸氧化酶活性成正比的荧光产物 (λex =540/λem = 590 nm) 。一单位谷氨酸氧化酶活性定义为：在室温和pH 7.4 条件下，每分钟催化L-谷氨酸转变为1.0 μmol α-酮戊二酸所需的酶量。
谷氨酸氧化酶检测试剂盒可简单、直接地测定溶液和细胞提取物等多种样品的谷氨酸氧化酶活性。该试剂盒通过酶偶联法测定谷氨酸氧化酶的活性，谷氨酸的氧化将产生与谷氨酸氧化酶活性成正比的荧光产物 (λex =540/λem = 590 nm) 。一单位谷氨酸氧化酶活性定义为：在室温和pH 7.4 条件下，每分钟催化L-谷氨酸转变为1.0 μmol α-酮戊二酸所需的酶量。
应用
Suitable for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts.
生化/生理作用
The glutamate oxidase assay kit provides a simple and direct procedure for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts. Glutamate oxidase activity is determined by a coupled enzyme assay, in which the oxidation of glutamic acid results in a fluorescent product (λex =540/λem = 590 nm) proportional to the glutamate oxidase activity present. One unit of glutamate oxidase activity is defined as the amount of enzyme that catalyzes the conversion of L-glutamic acid to 1.0μmole α-ketoglutaric acid per minute at pH 7.4 at room temperature.
警示用语：
Danger
危险声明
危险分类
Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1
储存分类代码
10 - Combustible liquids
法规信息
常规特殊物品
此项目有
Purification and properties of a new enzyme, l-glutamate oxidase, from Streptomyces sp. X-119-6 grown on wheat bran.
Kusakabe H, et al.
Agricultural and Biological Chemistry, 47(6), 1323-1328 (1983)
Jiro Arima et al.
The FEBS journal, 276(14), 3894-3903 (2009-06-18)
L-Glutamate oxidase (LGOX) from Streptomyces sp. X-119-6, which catalyzes the oxidative deamination of L-glutamate, has attracted increasing attention as a component of amperometric L-glutamate sensors used in the food industry and clinical biochemistry. The precursor of LGOX, which has a
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