Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric)

Angiotensin II converting enzyme (ACE2), a zinc-based metalloprotease is part of the renin-angiotensin system (RAS) that controls the regulation of blood pressure by cleaving the C-terminal dipeptide of Angiotensin II to convert it into Angiotensin 1-7. ACE2 is a receptor of human coronaviruses, such as SARS and HCoV-NL63. It is expressed on the vascular endothelial cells of lung, kidney and heart. ACE2 is a potential therapeutic target for cardiovascular and coronavirus-induced diseases.

The kit is suitable for the detection of ACE2 activity in tissue (for example lung, heart and kidney) and overexpressed ACE2 in cell lysates, as well as the determination of enzymatic activity of purified ACE2.

The Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit utilizes the ability of an active ACE2 to cleave a synthetic MCA based peptide substrate to release a free fluorophore. The released MCA can be easily quantified using a fluorescence microplate reader. An ACE2 specific inhibitor is also provided that can differentiate the ACE2 activity from other proteolytic activity. The kit is simple to use, can detect ACE2 concentrations as low as 0.4 mU.

The Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit is compatible with high-throughput handling systems.

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