Arginase Activity Colorimetric Assay Kit

The new Arginase Assay Kit, MAK533, is now available! Arginase is the final enzyme of the Urea Cycle. It converts L-arginine into urea and L-ornithine and plays an important role in removing ammonium ion from the body. Arginase has two isoforms: Arginase I and Arginase II. Arginase I is mainly present in the liver and plays an important part in the urea cycle whereas Arginase II is present in kidney and other tissues and regulates. Arginine/Ornithine concentration. Arginase deficiency can lead to severe symptoms including neurological impairment, dementia and hyperammonemia. Analysis of Arginase activity is fundamental to the study of the urea metabolic pathway.

The kit is suitable for the determination of arginase activity in tissues (liver, heart, kidney, etc.) and from cell culture (adherent and suspension cells).

The Arginase Activity Colorimetric Assay kit is simple, sensitive and rapid. In this assay, Arginase reacts with arginine & undergoes a series of reactions to form an intermediate that reacts stoichiometrically with the Probe to generate the colored product (A₅₇₀ nm). The kit can detect Arginase activity less than 1mU/well.

Arginase Activity Assay Kit:

• Sensitive, detecting Arginase activity less than 1mU/well.