Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric)

Carbonic anhydrases (CA) are zinc enzymes present in both prokaryotes and eukaryotes. They catalyze efficiently the reversible hydration of CO2 to bicarbonate. Their important patho-physiological roles in respiration, pH and CO2 homeostasis, secretion, gluconeogenesis, ureagenesis etc. make it an important drug target. Characteristic increase and decrease of CA Activity is observed in different physiological conditions and diseases such as anemia, Thalassemia, Hypothyroidism, Hyperthyroidism, and in several cases of lung and liver diseases and Leukemia.

The kit is suitable for the detection of carbonic anhydrase activity in hemolysates and serum, as well as the determination of enzymatic activity of purified CA.

The assay utilizes the esterase activity of an active CA on an ester substrate which releases a chromophore.The released product can be easily quantified using a spectrophotometric multi-well plate reader. In the presence of a CA specific inhibitor, the enzyme loses its activity which results in a decrease of absorbance.To measure specific CA activity, the CA inhibitor Acetazolamide is included.Acetazolamide is a potent inhibitor of CA I, CA II, CA IV, CA IX, CA XII etc. except CA III.

Carbonic Anhydrase (CA) Activity Assay Kit:

• Compatible with high-throughput handling systems.