Urea Colorimetric Assay Kit II

The new Urea Assay Kit, MAK471, now available!
Urea is a waste product which is produced in the liver, dissolved and transported in the blood (in a concentration of 1.6-7.5 mM), and excreted by the kidney. Urea plays a very important role in protein catabolism, removal of toxic ammonia from the body, and the countercurrent system, which allows for reabsorption of water and critical ions in the nephrons. Urea concentration is an important indicator for assessing the function of the kidneys and other organs.

The kit is suitable for the measurement of urea levels in biological samples (urine, serum, plasma etc.), tissue (liver, kidney etc.), adherent or suspension cells (3T3 cells, Jurkat cells etc.) and milk. It is also suitable for the analysis of liver and kidney function.

The Urea Colorimetric Assay Kit II is based on Jung′s method with a modification that delivers more robust and sensitive data. The urea is condensed with o-phthalaldehyde (OPA), followed by a reaction to form a colored product with strong absorbance at A₅₀₅ nm.

Urea Colorimetric Assay Kit II:

• Simple procedure with results in ∼1hour.
• Sensitive, detecting less than 10 μM of urea.