Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric)

Phosphoglycerate Dehydrogenase (PHGDH) is the first enzyme that participates in the L-serine synthesis pathway. PHGDH catalyzes the reversible reaction that converts 3-phosphoglycerate into 3 phosphohydroxyglycerate and the NADH-dependent reduction of a-ketoglutarate into D-2-hydroxyglutarate (D-2HG). In humans, PHGDH deficiency causes severe health problems including congenital microcephaly, psychomotor retardation, and intractable seizure. Recent studies have shown increased PHGDH activity in a wide variety of cancers, particularly in breast and melanoma tumors. Therefore, the measurement of PHGDH activity has become of great importance in searching potential therapeutic agents against cancer.

The kit is suitable for the measurement of PHGDH activity in various tissues (liver and kidney, etc.) and cells (adherent or suspension cells) and for the analysis of de novo L-serine biosynthesis.

The Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay kit provides a quick and easy method for monitoring PHGDH activity in a variety of samples. In this kit, phosphoglycerate dehydrogenase converts 3-phosphoglycerate and NAD into 3-phosphohydroxyglerate and NADH respectively. The oxidation of NADH reduces a probe generating a strong, stable absorbance signal at A₄₅₀ nm.

Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit:

• Compatible with high-throughput handling systems.
• Includes Positive Control.
• Sensitive, detecting phosphoglycerate dehydrogenase activity less than 0.1 mU per sample.