Citrulline Fluorometric Assay Kit

L-Citrulline is a non-proteogenic, semi-essential amino acid. Citrulline is formed either by ornithine carbamoyltransferase or as a by-product of the Nitric oxide synthase (NOS) activity. Citrulline generated in the NOS reaction can be recycled to arginine by the two enzymes argininosuccinate synthetase (ASS) and argininosuccinate lyase (ASL) acting in the Urea Cycle. Citrulline concentrations have been shown to be elevated in patients with mutated ASS or ASL genes. Watermelon is one of the richest sources of citrulline. Additionally, citrulline has been advertised as a sports nutrition supplement due to its involvement in Nitric Oxide (NO) synthesis, which helps with vasodilation.

The kit is suitable for the measurement of citrulline in beverages and biological samples (such as serum) and for the analysis of the urea cycle and NO cycle.

In this enzymatic assay, citrulline is converted into a series of intermediates, which further reacts with a probe producing a stable fluorescent signal (λ_Ex = 535 nm/λ_Em = 587 nm).

Citrulline Fluorometric Assay Kit:

• Compatible with high-throughput handling systems.
• Sensitive, detecting as low as 2 μM Citrulline in biological samples.