Mannitol Assay Kit

D-mannitol is a sugar alcohol used in dietary supplements, sweetener, intestinal permeability test for leaky gut, etc. It also serves as a coating for hard candies, dried fruits, and chewing gums due to its low ability to attract and hold water molecules. In addition, it is an osmoprotectant for plants and is used clinically in osmotherapy to reduce intracranial pressure.

The kit is suitable for mannitol determination in food, beverages, agricultural products, and biological samples such as urine and serum.

The Mannitol Assay Kit is based on the mannitol dehydrogenase catalyzed oxidation of D-mannitol, which generates D-fructose and NADH which reduces a formazan (MTT) dye. The intensity of product color, measured at 565 nm is directly proportional to D-mannitol concentration in the sample.