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Merck
CN

MOD50

Imprint® DNA Modification Kit

For bisulfite DNA conversion & purification

别名:

Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification

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关于此项目

EC 号:
231-548-0
UNSPSC代码:
41116158
NACRES:
NA.54

主要文件

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质量水平

200

用途

sufficient for 50 reactions

储存温度

20-25°C

相关类别

一般描述

印记®DNA修饰试剂盒包含完成亚硫酸氢盐转化和随后纯化DNA样本所需的所有试剂。DNA经化学变性，使亚硫酸氢盐试剂与单链DNA特异性反应，从而使胞嘧啶脱氨基并产生尿嘧啶残基。转化的DNA 适用于多种下游应用，包括甲基化特异性PCR、甲基化测序、焦磷酸测序以及甲基化微阵列。

应用

采用印迹®DNA修饰试剂盒进行DNA的亚硫酸氢修改。

特点和优势

  • 只需要50微微克的DNA或20个细胞
  • 整个流程用时不到2小时
  • 转化率大于99%
  • 降解率极低
  • 便捷一步协议选项
  • 一致且可重复的亚硫酸氢钠转换
  • 可用于基因组、内切核酸酶消化和FFPE DNA

制备说明

所有组分均可在室温下储存。每瓶DNA修饰粉末足以进行10次DNA修饰。溶解后，溶液可在-20°C下避光保存一周。使用前，必须在室温下解冻冷冻溶液并涡旋2分钟。

法律信息

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • T3566Clear-view™ Snap-Cap microtubes, size 1.5 mL, natural化学品安全说明书

象形图

CorrosionExclamation mark
GHS05,GHS07

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

补充剂危害

EUH031

储存分类代码

8B - Non-combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

危险化学品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000333642
0000333642
SLCQ0447
SLCD4557
SLCD4559

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如果您需要特殊版本，可通过批号或批次号查找具体证书。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How many reactions can be run with Product MOD50, Imprint® DNA Modification Kit?

    This kit can be used for 50 reactions.

  4. What is the minimum amount of DNA that can be used with Product MOD50, Imprint® DNA Modification Kit?

    DNA amounts ranging from 0.1 ng to 1 mg have been used successfully. For optimal modification, the DNA quantity should be in the range of 50-200 ng.

  5. Can I use Product MOD50, Imprint® DNA Modification Kit with DNA templates that contain high GC content or secondary structures?

    Yes, but it will be necessary to increase the bisulfite reaction time to 150 to 180 min.

  6. Can you store the DNA modified using Product MOD50, Imprint® DNA Modification Kit?

    The modified DNA generated with this kit can be stored for 2 months at -20 °C, 6 months at -80 °C.

  7. Can Product MOD50, Imprint® DNA Modification Kit, be used on formalin-fixed tissue ?

    Yes, it can be used on formalin-fixed tissue, but one would need to amplify the gene of interest before bisulfite treatment

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

David E Condon et al.
BMC bioinformatics, 19(1), 31-31 (2018-02-07)
Identification of differentially methylated regions (DMRs) is the initial step towards the study of DNA methylation-mediated gene regulation. Previous approaches to call DMRs suffer from false prediction, use extreme resources, and/or require library installation and input conversion. We developed a
Hwan Young Lee et al.
International journal of legal medicine, 126(1), 55-62 (2011-06-01)
DNA analysis of various body fluid stains at crime scenes facilitates the identification of individuals but does not currently determine the type and origin of the biological material. Recent advances in whole genome epigenetic analysis indicate that chromosome pieces called
Elena Ivanova et al.
Clinical epigenetics, 12(1), 64-64 (2020-05-13)
Preimplantation embryos experience profound resetting of epigenetic information inherited from the gametes. Genome-wide analysis at single-base resolution has shown similarities but also species differences between human and mouse preimplantation embryos in DNA methylation patterns and reprogramming. Here, we have extended
Nelly Olova et al.
Genome biology, 19(1), 33-33 (2018-03-17)
Whole-genome bisulfite sequencing (WGBS) is becoming an increasingly accessible technique, used widely for both fundamental and disease-oriented research. Library preparation methods benefit from a variety of available kits, polymerases and bisulfite conversion protocols. Although some steps in the procedure, such
Priyadarshini Kachroo et al.
Epigenomics, 10(2), 133-147 (2018-01-16)
To determine whether methylation differences between mostly fatal TCF3-HLF and curable TCF3-PBX1 pediatric acute lymphoblastic leukemia subtypes can be associated with differential gene expression and remission. Five (extremely rare) TCF3-HLF versus five (very similar) TCF3-PBX1 patients were sampled before and
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商品

DNA Methylation & Bisulfite Conversion

Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

实验方案

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

相关内容

Imprint ® DNA Modification Kit (MOD50) Protocol

The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.

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