MOD50
Imprint® DNA Modification Kit
For bisulfite DNA conversion & purification
别名:
Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification
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关于此项目
UNSPSC Code:
41116158
EC Number:
231-548-0
NACRES:
NA.54
General description
印记®DNA修饰试剂盒包含完成亚硫酸氢盐转化和随后纯化DNA样本所需的所有试剂。DNA经化学变性，使亚硫酸氢盐试剂与单链DNA特异性反应，从而使胞嘧啶脱氨基并产生尿嘧啶残基。转化的DNA 适用于多种下游应用，包括甲基化特异性PCR、甲基化测序、焦磷酸测序以及甲基化微阵列。
Application
采用印迹®DNA修饰试剂盒进行DNA的亚硫酸氢修改。
Features and Benefits
- 只需要50微微克的DNA或20个细胞
- 整个流程用时不到2小时
- 转化率大于99%
- 降解率极低
- 便捷一步协议选项
- 一致且可重复的亚硫酸氢钠转换
- 可用于基因组、内切核酸酶消化和FFPE DNA
Preparation Note
所有组分均可在室温下储存。每瓶DNA修饰粉末足以进行10次DNA修饰。溶解后，溶液可在-20°C下避光保存一周。使用前，必须在室温下解冻冷冻溶液并涡旋2分钟。
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
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Danger
supp_hazards
存储类别
8B - Non-combustible corrosive hazardous materials
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
法规信息
危险化学品
此项目有
The elephant shark methylome reveals conservation of epigenetic regulation across jawed vertebrates
Peat JR, et al.
F1000Research (2017)
Sarah Rose Huffman et al.
Methods in molecular biology (Clifton, N.J.), 925, 201-209 (2012-08-22)
Researchers whose experimental models are mammalian oocytes and preimplantation embryos are often limited by the yield of nucleic acids that can be isolated from such a small sample size. In addition, the limited number of cells from these types of
Julian R Peat et al.
Methods in molecular biology (Clifton, N.J.), 1708, 161-169 (2017-12-11)
The epigenetic mark 5-methylcytosine confers heritable regulation of gene expression that can be dynamically modulated during transitions in cell fate. With the development of high-throughput sequencing technologies, it is now possible to obtain comprehensive genome-wide maps of the mammalian DNA
M Y Follo et al.
Leukemia, 26(5), 943-950 (2011-10-29)
Phosphoinositide-phospholipase C (PI-PLC) beta1 can be considered a specific target for demethylating therapy in high-risk myelodysplastic syndrome (MDS) patients, as azacitidine treatment has been associated with a PI-PLCbeta1-specific promoter demethylation, and induction of PI-PLCbeta1 gene and protein expression. However, little
Susanne Edelmann et al.
Methods in molecular biology (Clifton, N.J.), 1675, 45-60 (2017-10-21)
Bisulfite sequencing (BS-seq) enables the detection of DNA methylation at cytosine residues (5mC) at single-nucleotide resolution. For many applications, a limiting factor of conventional BS-seq protocols is the high amount of DNA required, since the treatment with bisulfite causes severe
商品
Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.
实验方案
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
相关内容
The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MOD50-1KT | 04061834066804 |
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