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Merck
CN

MOD50

Sigma-Aldrich

Imprint® DNA Modification Kit

For bisulfite DNA conversion & purification

别名:

Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification

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关于此项目

EC 号:
UNSPSC代码:
41116158
NACRES:
NA.54
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质量水平

用途

sufficient for 50 reactions

储存温度

20-25°C

相关类别

一般描述

印记®DNA修饰试剂盒包含完成亚硫酸氢盐转化和随后纯化DNA样本所需的所有试剂。DNA经化学变性,使亚硫酸氢盐试剂与单链DNA特异性反应,从而使胞嘧啶脱氨基并产生尿嘧啶残基。转化的DNA 适用于多种下游应用,包括甲基化特异性PCR、甲基化测序、焦磷酸测序以及甲基化微阵列。

应用

采用印迹®DNA修饰试剂盒进行DNA的亚硫酸氢修改。

特点和优势

  • 只需要50微微克的DNA或20个细胞
  • 整个流程用时不到2小时
  • 转化率大于99%
  • 降解率极低
  • 便捷一步协议选项
  • 一致且可重复的亚硫酸氢钠转换
  • 可用于基因组、内切核酸酶消化和FFPE DNA

制备说明

所有组分均可在室温下储存。每瓶DNA修饰粉末足以进行10次DNA修饰。溶解后,溶液可在-20°C下避光保存一周。使用前,必须在室温下解冻冷冻溶液并涡旋2分钟。

法律信息

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • T3566Clear-view Snap-Cap microtubes, size 1.5 mL, natural化学品安全说明书

象形图

CorrosionExclamation mark

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

补充剂危害

储存分类代码

8B - Non-combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

危险化学品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How many reactions can be run with Product MOD50, Imprint® DNA Modification Kit?

    This kit can be used for 50 reactions.

  4. What is the minimum amount of DNA that can be used with Product MOD50, Imprint® DNA Modification Kit?

    DNA amounts ranging from 0.1 ng to 1 mg have been used successfully. For optimal modification, the DNA quantity should be in the range of 50-200 ng.

  5. Can I use Product MOD50, Imprint® DNA Modification Kit with DNA templates that contain high GC content or secondary structures?

    Yes, but it will be necessary to increase the bisulfite reaction time to 150 to 180 min.

  6. Can you store the DNA modified using Product MOD50, Imprint® DNA Modification Kit?

    The modified DNA generated with this kit can be stored for 2 months at -20 °C, 6 months at -80 °C.

  7. Can Product MOD50, Imprint® DNA Modification Kit, be used on formalin-fixed tissue ?

    Yes, it can be used on formalin-fixed tissue, but one would need to amplify the gene of interest before bisulfite treatment

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Susanne Edelmann et al.
Methods in molecular biology (Clifton, N.J.), 1675, 45-60 (2017-10-21)
Bisulfite sequencing (BS-seq) enables the detection of DNA methylation at cytosine residues (5mC) at single-nucleotide resolution. For many applications, a limiting factor of conventional BS-seq protocols is the high amount of DNA required, since the treatment with bisulfite causes severe
Sarah Rose Huffman et al.
Methods in molecular biology (Clifton, N.J.), 925, 201-209 (2012-08-22)
Researchers whose experimental models are mammalian oocytes and preimplantation embryos are often limited by the yield of nucleic acids that can be isolated from such a small sample size. In addition, the limited number of cells from these types of
Julian R Peat et al.
Methods in molecular biology (Clifton, N.J.), 1708, 161-169 (2017-12-11)
The epigenetic mark 5-methylcytosine confers heritable regulation of gene expression that can be dynamically modulated during transitions in cell fate. With the development of high-throughput sequencing technologies, it is now possible to obtain comprehensive genome-wide maps of the mammalian DNA
M Y Follo et al.
Leukemia, 26(5), 943-950 (2011-10-29)
Phosphoinositide-phospholipase C (PI-PLC) beta1 can be considered a specific target for demethylating therapy in high-risk myelodysplastic syndrome (MDS) patients, as azacitidine treatment has been associated with a PI-PLCbeta1-specific promoter demethylation, and induction of PI-PLCbeta1 gene and protein expression. However, little
The elephant shark methylome reveals conservation of epigenetic regulation across jawed vertebrates
Peat JR, et al.
F1000Research (2017)

商品

Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

实验方案

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

相关内容

The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.

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