MOD50
Imprint® DNA Modification Kit
For bisulfite DNA conversion & purification
别名:
Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification
一般描述
应用
特点和优势
- 只需要50微微克的DNA或20个细胞
- 整个流程用时不到2小时
- 转化率大于99%
- 降解率极低
- 便捷一步协议选项
- 一致且可重复的亚硫酸氢钠转换
- 可用于基因组、内切核酸酶消化和FFPE DNA
制备说明
法律信息
警示用语:
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
补充剂危害
储存分类代码
8B - Non-combustible corrosive hazardous materials
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
历史批次信息供参考:
分析证书(COA)
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How many reactions can be run with Product MOD50, Imprint® DNA Modification Kit?
This kit can be used for 50 reactions.
What is the minimum amount of DNA that can be used with Product MOD50, Imprint® DNA Modification Kit?
DNA amounts ranging from 0.1 ng to 1 mg have been used successfully. For optimal modification, the DNA quantity should be in the range of 50-200 ng.
Can I use Product MOD50, Imprint® DNA Modification Kit with DNA templates that contain high GC content or secondary structures?
Yes, but it will be necessary to increase the bisulfite reaction time to 150 to 180 min.
Can you store the DNA modified using Product MOD50, Imprint® DNA Modification Kit?
The modified DNA generated with this kit can be stored for 2 months at -20 °C, 6 months at -80 °C.
Can Product MOD50, Imprint® DNA Modification Kit, be used on formalin-fixed tissue ?
Yes, it can be used on formalin-fixed tissue, but one would need to amplify the gene of interest before bisulfite treatment
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.
实验方案
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
相关内容
The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.
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