用途
sufficient for 25 reactions
质量
Not suitable for clinical diagnostic use.
技术
PCR: suitable
应用
detection
microbiology
储存温度
2-8°C
一般描述
The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals.
The primer/probe system detects the highly conserved 16S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.
This kit has been tested and optimized for use with JumpStartä Taq DNA Polymerase, Catalog Number D9307. It is highly recommended that this product be used with the kit. Use of other Taq polymerase products may require reaction optimization to achieve proper results.
The primer/probe system detects the highly conserved 16S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.
This kit has been tested and optimized for use with JumpStartä Taq DNA Polymerase, Catalog Number D9307. It is highly recommended that this product be used with the kit. Use of other Taq polymerase products may require reaction optimization to achieve proper results.
应用
qPCR Probe Kit for high quality and reliable quantitative detection of mycoplasma DNA in research, industrial application and product testing. Usable for direct testing of cell cultures and biologicals in combination with cell culture enrichment.
法律信息
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
闪点(°F)
188.6 °F
闪点(°C)
87 °C
法规信息
新产品
此项目有
Young Yi et al.
Cancers, 13(18) (2021-09-29)
Osteosarcoma, the most common primary bone malignancy, is typically related to growth spurts during adolescence. Prognosis is very poor for patients with metastatic or recurrent osteosarcoma, with survival rates of only 20-30%. Epithelial-mesenchymal transition (EMT) is a cellular mechanism that
Qinglei Kong et al.
OncoTargets and therapy, 11, 6489-6503 (2018-10-17)
MicroRNA (miR)-431 plays an essential role in various human cancer types, particularly in the process of invasion. However, the function and mechanism of miR-431-5p in the invasion of hepatocellular carcinoma (HCC) remain undefined. The expression levels of miR-431-5p and its
Hee Eun Kang et al.
Cancers, 13(14) (2021-07-25)
The Wnt and Hippo pathways are tightly coordinated and understanding their reciprocal regulation may provide a novel therapeutic strategy for cancer. Anti-helminthic niclosamide is an effective inhibitor of Wnt and is now in a phase II trial for advanced colorectal
Nicola Aceto et al.
Molecular cancer research : MCR, 16(4), 720-727 (2018-02-18)
Molecular drivers underlying bone metastases in human cancer are not well understood, in part due to constraints in bone tissue sampling. Here, RNA sequencing was performed of circulating tumor cells (CTC) isolated from blood samples of women with metastatic estrogen
Renxu Chang et al.
Nature communications, 9(1), 3486-3486 (2018-08-30)
Loss of WW domain-containing oxidoreductase (Wwox) expression has been observed in breast cancer (BC). However, its regulatory effects are largely unknown, especially in triple-negative breast cancer (TNBC). Herein, gene expression profiling revealed that JAK/STAT3 pathway was one of the most
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持