N0790
NDSB 221
≥97% (TLC)
别名:
3-(1-Methylpiperidinio)-1-propanesulfonate, 3-(1-Methylpiperidinium)-1-propane sulfonate
description
zwitterionic
assay
≥97% (TLC)
form
solid
mol wt
221.32 g/mol
color
white
solubility
H2O: 50 mg/mL, clear, colorless
General description
NDSB 221 is a nondetergent sulfobetaine that has been found to improve unfolding reversibility.
Application
NDSB 221 has been used in a study to identify a novel ligand binding site in phosphoserine phosphatase from Thermococcus onnurineus. It has also been used in a study to investigate its effects on acidic fibroblast growth factor (aFGF).
Non-detergent sulfobetaine is a compound used for non-denaturing protien purification. Increases the extraction yield of membrane, nuclear and cytoskeletal associated proteins. Zwitterionic over a wide pH range, easily removed by dialysis and no significant absorption in the near UV range. Specific applications include microsomal protein extraction, nuclear protein recovery, precipitation reduction in IEF, and membrane-bound protease purification. The typical usage concentration is 0.5 - 2.0 M.
Other Notes
This product is non-micelle forming.
signalword
Danger
hcodes
Hazard Classifications
Skin Corr. 1B
存储类别
8A - Combustible corrosive hazardous materials
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
A nondetergent sulfobetaine improves protein unfolding reversibility in microcalorimetric studies
Salvino S D'Amico and Georges G Feller
Analytical Biochemistry, 385, 3-3 (2009)
Identification of a novel ligand binding site in phosphoserine phosphatase from the hyperthermophilic archaeon Thermococcus onnurineus
Tae-Yang Jung et al.
Proteins: Structure, Function, and Genetics, 819-829 (2012)
Long Xiang et al.
Journal of magnetic resonance (San Diego, Calif. : 1997), 194(1), 147-151 (2008-07-12)
Prevention of aggregation is critical for analyzing protein structure. Non-detergent sulfobetaines (NDSBs) are known to prevent protein aggregation, but the molecular mechanisms of their anti-aggregation effect are poorly understood. To elucidate the underlying mechanisms, we analyzed the effects of dimethylethylammonium
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