N1533
一氧化氮合酶，Endothelial 牛
recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous solution
别名:
NOS III, ecNOS
recombinant
expressed in baculovirus infected Sf9 cells
form
buffered aqueous solution
specific activity
≥0.3 units/mg protein
mol wt
135 (kDa/subunit, homodimer)
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
cow ... NOS3(287024)
Biochem/physiol Actions
NOS负责L-精氨酸生物合成一氧化氮，并组成性表达于内皮细胞（eNOS）和大脑（nNOS）中。
用于在外源添加的辅因子和/或抑制剂存在下测量NOS的活性。
负责L-精氨酸生物合成一氧化氮；组成性表达于内皮细胞（eNOS）和大脑（nNOS）中。
适用于在外源添加的辅因子和/或抑制剂存在下测量NOS的活性。
Physical form
溶于含有10%甘油、5 mM CHAPS和100 μM DTT的50 mM HEPES，pH 7.4的溶液。
Other Notes
在含有5 μM氧合血红蛋白、1 mM CaCl2、20 μg/ml钙调蛋白、0.1 mM NADPH、50 μM精氨酸、12 μM四氢生物蝶呤和170 μM DTT的50 mM HEPES，pH7.4中，一单位的酶在37 °C每分钟可产生1 nmole的一氧化氮。
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Ying-Ling Lai et al.
Journal of clinical biochemistry and nutrition, 50(1), 84-89 (2012-01-17)
The cholesterol ozonolysis products secosterol-A and its aldolization product secosterol-B were recently detected in human atherosclerotic tissues and brain specimens, and have been postulated to play pivotal roles in the pathogenesis of atherosclerosis and neurodegenerative diseases. We examined several oxidized
Silviya R Stateva et al.
PloS one, 10(4), e0120798-e0120798 (2015-04-02)
Calmodulin (CaM) phosphorylated at different serine/threonine and tyrosine residues is known to exert differential regulatory effects on a variety of CaM-binding enzymes as compared to non-phosphorylated CaM. In this report we describe the preparation and characterization of a series of
Inhibition of L-arginine metabolizing enzymes by L-arginine-derived advanced glycation end products.
Ying-Ling Lai et al.
Journal of clinical biochemistry and nutrition, 46(2), 177-185 (2010-03-11)
N(omega)-Carboxymethyl-arginine (CMA), N(omega)-carboxyethyl-arginine (CEA) and N(delta)-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine (MG-H1) have been identified as L-arginine-derived advanced glycation end products (AGEs) formed by non-enzymatic reactions between reducing sugars such as glucose and amino groups in proteins. These AGEs are structurally analogous to endogenous inhibitors
Heather S Bevan et al.
American journal of physiology. Renal physiology, 301(4), F733-F742 (2011-07-22)
Laminar shear stress is a key determinant of systemic vascular behavior, including through activation of endothelial nitric oxide synthase (eNOS), but little is known of its role in the glomerulus. We confirmed eNOS expression by glomerular endothelial cells (GEnC) in
Yunzhou Dong et al.
Journal of cellular and molecular medicine, 13(9A), 2899-2910 (2008-07-16)
Oxidation and glycation of low-density lipoprotein (LDL) promote vascular injury in diabetes; however, the mechanisms underlying this effect remain poorly defined. The present study was conducted to determine the effects of 'heavily oxidized' glycated LDL (HOG-LDL) on endothelial nitric oxide
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