重组
expressed in baculovirus infected Sf9 cells
方案
≥95%
表单
buffered aqueous solution
比活
≥200 units/mg protein
分子量
150 (kDa/subunit, homodimer)
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
rat ... Nos1(24598)
生化/生理作用
NOS is responsible for the biosynthesis of nitric oxide from L-arginine. nNOS is calcium/calmodulin dependent and has a Km = 2.0 μM for L-arginine.
单位定义
One unit of enzyme produces 1 nmole of nitric oxide per minute at 37 °C in 50 mM HEPES, pH 7.4, containing 5 μM oxyhemoglobin, 1 mM CaCl2, 20 μg/ml calmodulin, 0.1 mM NADPH, 50 μM arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.
外形
Solution in 50 mM HEPES, pH 7.4, with 20% glycerol, 100 mM NaCl and 10 μM tetrahydrobiopterin.
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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