N3755
微球菌核酸酶 来源于金黄色葡萄球菌
100-300 units/mg protein
别名:
微球菌核酸酶, MNase, 微球菌核酸内切酶
产品名称
微球菌核酸酶 来源于金黄色葡萄球菌, 100-300 units/mg protein
biological source
Staphylococcus aureus
form
lyophilized powder
specific activity
100-300 units/mg protein
technique(s)
DNA purification: suitable
suitability
suitable for molecular biology
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Quality Level
Gene Information
Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)
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Application
来自 金黄色葡萄球菌 的核酸酶已被用于评估动物中发现的凝固酶和耐热菌株的研究中。 它也被用于研究 金黄色葡萄球菌 中编码两个热稳定核酸酶基因的表达特征。
Biochem/physiol Actions
可水解DNA的5′-磷酸二酯键。
相较于G或C侧，微球菌核酸酶可在A或T核苷酸的5′ 侧好的对DNA进行切割。
Other Notes
在pH 8.8，37℃条件下，一个单元可每分钟从天然DNA产生1.0μmole的酸可溶性多核苷酸，对于混合核苷酸来说基于EM/260 = 10,000 。
注意：1μmole单位 = 约85 A260 单位，其中A260单位相当于在pH 8.8，37℃条件下，在3.55 mL反应体积中，30分钟内ΔA260为1.0。光路= 1cm。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Coagulase and Heat-resistant Nuclease Producing Staphylococcus epidermidis Strains from Animals
DEVRIESE, L. and P. OEDING
Journal of Applied Microbiology, 39, 197-207 (1975)
C Dingwall et al.
Nucleic acids research, 9(12), 2659-2673 (1981-06-25)
The substrate specificity of micrococcal nuclease (EC 3.1.4.7.) has been studied. The enzyme recognises features of nucleotide composition, nucleotide sequence and tertiary structure of DNA. Kinetic analysis indicates that the rate of cleavage is 30 times greater at the 5'
Yu Hu et al.
Foodborne pathogens and disease, 9(3), 265-271 (2012-03-03)
Thermonuclease is known as a specific virulence factor in Staphylococcus aureus. It is widely used as a genetic marker for detection of S. aureus in various types of food. Previous studies have revealed the existence of two functional thermostable nucleases
Frank J Hernandez et al.
Nature medicine, 20(3), 301-306 (2014-02-04)
Technologies that enable the rapid detection and localization of bacterial infections in living animals could address an unmet need for infectious disease diagnostics. We describe a molecular imaging approach for the specific, noninvasive detection of S. aureus based on the
Joana Segura et al.
Nature communications, 9(1), 3989-3989 (2018-09-30)
The interplay between chromatin structure and DNA topology is a fundamental, yet elusive, regulator of genome activities. A paradigmatic case is the "linking number paradox" of nucleosomal DNA, which refers to the incongruence between the near two left-handed superhelical turns
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