N3755
微球菌核酸酶 来源于金黄色葡萄球菌
100-300 units/mg protein
别名:
微球菌核酸酶, MNase, 微球菌核酸内切酶
生物来源
Staphylococcus aureus
质量水平
表单
lyophilized powder
比活
100-300 units/mg protein
组成
Protein, ≥40% E1%/280 (, Balance primarily sodium citrate)
技术
DNA purification: suitable
适用性
suitable for molecular biology
应用
diagnostic assay manufacturing
储存温度
−20°C
基因信息
Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)
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应用
来自 金黄色葡萄球菌 的核酸酶已被用于评估动物中发现的凝固酶和耐热菌株的研究中。 它也被用于研究 金黄色葡萄球菌 中编码两个热稳定核酸酶基因的表达特征。
生化/生理作用
可水解DNA的5′-磷酸二酯键。
相较于G或C侧，微球菌核酸酶可在A或T核苷酸的5′ 侧好的对DNA进行切割。
其他说明
在pH 8.8，37℃条件下，一个单元可每分钟从天然DNA产生1.0μmole的酸可溶性多核苷酸，对于混合核苷酸来说基于EM/260 = 10,000 。
注意：1μmole单位 = 约85 A260 单位，其中A260单位相当于在pH 8.8，37℃条件下，在3.55 mL反应体积中，30分钟内ΔA260为1.0。光路= 1cm。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Coagulase and Heat-resistant Nuclease Producing Staphylococcus epidermidis Strains from Animals
DEVRIESE, L. and P. OEDING
Journal of Applied Microbiology, 39, 197-207 (1975)
Joana Segura et al.
Nature communications, 9(1), 3989-3989 (2018-09-30)
The interplay between chromatin structure and DNA topology is a fundamental, yet elusive, regulator of genome activities. A paradigmatic case is the "linking number paradox" of nucleosomal DNA, which refers to the incongruence between the near two left-handed superhelical turns
Frank J Hernandez et al.
Nature medicine, 20(3), 301-306 (2014-02-04)
Technologies that enable the rapid detection and localization of bacterial infections in living animals could address an unmet need for infectious disease diagnostics. We describe a molecular imaging approach for the specific, noninvasive detection of S. aureus based on the
Julien Roche et al.
Journal of the American Chemical Society, 135(39), 14610-14618 (2013-08-31)
The time required to fold proteins usually increases significantly under conditions of high pressure. Taking advantage of this general property of proteins, we combined P-jump experiments with NMR spectroscopy to examine in detail the folding reaction of staphylococcal nuclease (SNase)
Yu Hu et al.
Foodborne pathogens and disease, 9(3), 265-271 (2012-03-03)
Thermonuclease is known as a specific virulence factor in Staphylococcus aureus. It is widely used as a genetic marker for detection of S. aureus in various types of food. Previous studies have revealed the existence of two functional thermostable nucleases
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