生物来源
Aspergillus sp. (A. oryzae)
质量水平
表单
solution
浓度
≥100000 units/mL
技术
DNA purification: suitable
适用性
suitable for nucleic acid purification
应用
cell analysis
运输
wet ice
储存温度
−20°C
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一般描述
来源于米曲霉的核酸酶 S1 酶具有降解由脱氧核苷酸或核糖核苷酸组成的单链寡核苷酸的能力。
应用
在一项研究中，来源于米曲霉的核酸酶 S1 被用于评估标测 DNA 突变的生物化学方法。在另一项研究中，它还被用于研究 γ-照射的大鼠脑肿瘤中的 DNA 损伤和修复。
生化/生理作用
从米曲霉分离的核酸酶 S1 具有对单链 DNA 和 RNA 磷酸二酯键的内切和外切水解活性，产生 5'-磷酸单核苷酸和 5'-磷酸寡核苷酸终产物。它用于消化 RNA 和 DNA 双链体中未退火的多核苷酸尾部和发夹环，并可用于将超螺旋 DNA 转化为线性形式。
来源于米曲霉的 SI 核酸酶能生成 DNA 断裂或脱碱基位点产生双链 DNA 断裂。
外形
含有 30mM 乙酸钠，50mM NaCl，1mM ZnCl2，50％ 甘油，2mg/ml 蛋白质的溶液
其他说明
在 37℃，pH4.6 条件下， 一单位 SI 核酸酶每分钟将 1.0 ug 的单链核酸转化成高氯酸。
仅试剂盒组分
产品编号
说明
- 30mM Sodium acetate .25-.25 %
- 50mM Sodium chloride .29 %
- 1mM Zinc chloride .01 %
- Glycerol 50 %
- 2mg/mL Protein .2 %
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
M A Chaudhry et al.
Nucleic acids research, 23(19), 3805-3809 (1995-10-11)
Defined DNA substrates containing discrete abasic sites or paired abasic sites set 1, 3, 5 and 7 bases apart on opposite strands were constructed to examine the reactivity of S1, mung bean and P1 nucleases towards abasic sites. None of
Rebecca Rodell et al.
Methods in molecular biology (Clifton, N.J.), 2444, 125-140 (2022-03-16)
Physiological and chemically induced modifications to nucleosides are common in both DNA and RNA. Physiological forms of these modifications play critical roles in gene expression, yet aberrant marks, if left unrepaired, may be associated with increased genome instability. Due to
Robert Busch et al.
Nature protocols, 2(12), 3045-3057 (2007-12-15)
DNA replication occurs almost exclusively during S-phase of the cell cycle and represents a simple biochemical metric of cell division. Previous methods for measuring cell proliferation rates have important limitations. Here, we describe experimental protocols for measuring cell proliferation and
P Beard et al.
Journal of virology, 12(6), 1303-1313 (1973-12-01)
S(1) nuclease, the single-strand specific nuclease from Aspergillus oryzae can cleave both strands of circular covalently closed, superhelical simian virus 40 (SV40) DNA to generate unit length linear duplex molecules with intact single strands. But circular, covalently closed, nonsuperhelical DNA
Joachim Kloehn et al.
Methods in molecular biology (Clifton, N.J.), 2116, 587-609 (2020-03-30)
This protocol describes the use of heavy water (2H2O) labeling to determine the growth rate and metabolic state of Leishmania parasites in culture and in infected animals. In vitro labeling studies are undertaken by cultivating defined parasite developmental stages in
实验方案
Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.
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