N5661
核酸酶 S1 来源于米曲霉
for single-strand DNA/RNA digestion
别名:
核酸内切酶 S1
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Biological source:
Aspergillus sp. (A. oryzae)
Concentration:
≥100000 units/mL
biological source
Aspergillus sp. (A. oryzae)
form
solution
concentration
≥100000 units/mL
technique(s)
DNA purification: suitable
suitability
suitable for nucleic acid purification
application(s)
cell analysis
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
来源于米曲霉的核酸酶 S1 酶具有降解由脱氧核苷酸或核糖核苷酸组成的单链寡核苷酸的能力。
Application
在一项研究中，来源于米曲霉的核酸酶 S1 被用于评估标测 DNA 突变的生物化学方法。在另一项研究中，它还被用于研究 γ-照射的大鼠脑肿瘤中的 DNA 损伤和修复。
Biochem/physiol Actions
从米曲霉分离的核酸酶 S1 具有对单链 DNA 和 RNA 磷酸二酯键的内切和外切水解活性，产生 5'-磷酸单核苷酸和 5'-磷酸寡核苷酸终产物。它用于消化 RNA 和 DNA 双链体中未退火的多核苷酸尾部和发夹环，并可用于将超螺旋 DNA 转化为线性形式。
来源于米曲霉的 SI 核酸酶能生成 DNA 断裂或脱碱基位点产生双链 DNA 断裂。
Physical form
含有 30mM 乙酸钠，50mM NaCl，1mM ZnCl2，50％ 甘油，2mg/ml 蛋白质的溶液
Other Notes
在 37℃，pH4.6 条件下， 一单位 SI 核酸酶每分钟将 1.0 ug 的单链核酸转化成高氯酸。
仅试剂盒组分
产品编号
说明
- 30mM Sodium acetate .25-.25 %
- 50mM Sodium chloride .29 %
- 1mM Zinc chloride .01 %
- Glycerol 50 %
- 2mg/mL Protein .2 %
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
S1 nuclease from Aspergillus oryzae for the detection of DNA damage and repair in the gamma-irradiated intracerebral rat gliosarcoma 9L.
P H Gutin et al.
Radiation research, 72(1), 100-106 (1977-10-01)
T E Shenk et al.
Proceedings of the National Academy of Sciences of the United States of America, 72(3), 989-993 (1975-03-01)
S1 nuclease (EC 3.1.4.X), a single-strand-specific nuclease, can be used to accurately map the location of mutational alterations in simian virus 40 (SV40) DNA. Deletions of between 32 and 190 base pairs, which are at or below the limit of
Rebecca Rodell et al.
Methods in molecular biology (Clifton, N.J.), 2444, 125-140 (2022-03-16)
Physiological and chemically induced modifications to nucleosides are common in both DNA and RNA. Physiological forms of these modifications play critical roles in gene expression, yet aberrant marks, if left unrepaired, may be associated with increased genome instability. Due to
Robert Busch et al.
Nature protocols, 2(12), 3045-3057 (2007-12-15)
DNA replication occurs almost exclusively during S-phase of the cell cycle and represents a simple biochemical metric of cell division. Previous methods for measuring cell proliferation rates have important limitations. Here, we describe experimental protocols for measuring cell proliferation and
M A Chaudhry et al.
Nucleic acids research, 23(19), 3805-3809 (1995-10-11)
Defined DNA substrates containing discrete abasic sites or paired abasic sites set 1, 3, 5 and 7 bases apart on opposite strands were constructed to examine the reactivity of S1, mung bean and P1 nucleases towards abasic sites. None of
实验方案
Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.
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