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Merck
CN

N8882

NAD+ Kinase from chicken liver

Type IV, lyophilized powder, 10-20 units/mg solid

别名:

ATP: NAD+ 2′-phosphotransferase

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化学文摘社编号:
UNSPSC Code:
12352204
MDL number:
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type

Type IV

form

lyophilized powder

specific activity

10-20 units/mg solid

composition

Protein, ≥80% biuret

UniProt accession no.

storage temp.

−20°C

Gene Information

chicken ... RCJMB04_9i6(419403)

Biochem/physiol Actions

Reaction: β-NAD + ATP → β-NADP + ADP.

Physical form

Lyophilized powder containing citrate buffer

Other Notes

One unit will phosphorylate 1.0 nanomole of β-NAD to β-NADP per min at pH 7.5 at 37 °C, in the presence of ATP.


存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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Lee Hunt et al.
The New phytologist, 181(1), 62-70 (2008-10-02)
To investigate the proposed role for NAD metabolism in regulating seed dormancy, NAD metabolites and associated enzyme activities were analysed in seed of Arabidopsis thaliana ecotypes ranging from Col-0, which has low seed dormancy, to Cvi, which is highly dormant.
Thomas Orsak et al.
Biochemistry, 51(1), 225-232 (2011-11-30)
Small molecule allostery modifies protein function but is not easily discovered. We introduce mass spectrometry integrated with equilibrium dialysis for the discovery of allostery systematically (MIDAS), a method for identifying physiologically relevant, low-affinity metabolite-protein interactions using unmodified proteins and complex
Hyeon Cheol Lee et al.
Biotechnology letters, 31(12), 1929-1936 (2009-09-24)
Abstract Intracellular NADPH/NADP+ ratio in cells grown on various production media with different carbon and nitrogen sources had a positive correlation with the thymidine production. To improve thymidine production in a previously engineered E. coli strain, NAD+ kinase was overexpressed