N9411
Nerve growth factor receptor/Fc chimera from mouse
recombinant, expressed in mouse NSO cells, lyophilized powder, suitable for cell culture
别名:
NGF R, p75 Neurotrophin Receptor
重组
expressed in mouse NSO cells
方案
≥90% (SDS-PAGE)
表单
lyophilized powder
分子量
apparent mol wt 75-100 kDa by SDS-PAGE (reducing)
calculated mol wt 50.4 kDa
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
UniProt登记号
储存温度
−20°C
基因信息
mouse ... Ngfr(18053)
生化/生理作用
The NGF receptor also named low-affinity NGF receptor (LNGFR), a potent NGF antagonist, is a 75 kDa, type I transmembrane protein consisting of an extracellular domain, which contains four cysteine-rich domains responsible for ligand binding. The cytoplasmic region of the receptor contains a subtype 2 death domain. Nerve Growth Factor Receptor (NGF R), binds NGF and other neurotrophins including BDNF, NT-3, and NT-4 with similar affinity. NGF-R is necessary for regulating neuronal growth, cell migration, gene expression, differentiation, and cell death during development of the central and peripheral nervous system.
外形
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.
制备说明
Nerve growth factor receptor/Fc chimera mouse is a chimeric protein expressed in a mouse myeloma cell line, NSO. A DNA sequence encoding the signal peptide from human CD33 joined with the extracellular domain of mouse nerve growth factor receptor (NGF R)1 is fused to the Fc region of human IgG1 via a peptide linker. Mature recombinant mouse NGF R /Fc is a disulfide linked homodimeric protein. Based on N-terminal amino acid sequencing, the mature protein starts at Gly 17. The recombinant mouse NGF R/Fc monomer has a calculated molecular mass 50.4 kDa. As a result of glycosylation, recombinant mouse NGF receptor/Fc migrates with an apparent molecular mass of 75-100 kDa in SDS-PAGE.
分析说明
The biological activity is measured by its ability to inhibit recombinant mouse β-NGF-induced proliferation of TF-1 cells.
储存分类代码
13 - Non Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
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S X Bamji et al.
The Journal of cell biology, 140(4), 911-923 (1998-03-21)
To determine whether the p75 neurotrophin receptor (p75NTR) plays a role in naturally occurring neuronal death, we examined neonatal sympathetic neurons that express both the TrkA tyrosine kinase receptor and p75NTR. When sympathetic neuron survival is maintained with low quantities
C Tuffereau et al.
The EMBO journal, 17(24), 7250-7259 (1998-12-19)
A random-primed cDNA expression library constructed from the mRNA of neuroblastoma cells (NG108) was used to clone a specific rabies virus (RV) receptor. A soluble form of the RV glycoprotein (Gs) was utilized as a ligand to detect positive cells.
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