N9527
Monoclonal Anti-NTF2 antibody produced in mouse
~2 mg/mL, clone 4F5, purified immunoglobulin, buffered aqueous solution
别名:
Anti-Nuclear Transfer Factor 2
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关于此项目
Conjugate:
unconjugated
Clone:
4F5, monoclonal
Application:
ARR, ELISA (i), ICC, IP, WB
Citations:
8
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4F5, monoclonal
form
buffered aqueous solution
mol wt
antigen 14 kDa
species reactivity
human, hamster, monkey, mouse, rat, chicken, Xenopus
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable using 3.7% formaldehyde-0.2% Triton X-100 or 4% paraformaldehyde-methanol, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using HeLa cells cytosol preparation
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
human ... NUTF2(10204)
mouse ... Ntf2(100043462)
rat ... Nutf2(291981)
General description
Nuclear transport factor 2 (NTF2 or NUTF2) is a cytosolic soluble protein. The polypeptide chain of NTF2 has α and β sheets and a hydrophobic cavity. NUTF2 is localized on the human chromosome on 16q22.1.
Immunogen
recombinant human NTF2. The epitope lies within the extreme C-terminus of NTF2.
Application
Monoclonal Anti-NTF2 antibody produced in mouse has been used in western blotting.
Biochem/physiol Actions
Nuclear transport factor 2 (NTF2 or NUTF2) binds with Ras (Rat Sarcoma)-family GTPase (guanosine triphosphatase) Ran (Ras related nuclear protein) and facilitates the transport of Ran to the nucleus. NTF2 binds to nuclear pore complex (NPC) nucleoporin proteins with FxFG repeats especially p62 also binds preferably to GDP-Ran rather than GTP-Ran to form the complex GDP-Ran and p62 binds at different regions in the hydrophobic cavity of NTF2. The mutation in NTF2 hinders the binding and transport of Ran and formation the p62-NTF2-Ran complex.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Analysis Note
The antibody may also be used for microinjection, in vitro nuclear protein import assays, and blocking of NTF2 binding to Ran.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Fine mapping of 16q-linked autosomal dominant cerebellar ataxia type III in Japanese families
Hirano R, et al.
Neurogenetics, 5(4), 215-221 (2004)
The 1.6 angstrom resolution crystal structure of nuclear transport factor 2 (NTF2)
Bullock TL, et al.
Journal of Molecular Biology, 260(3), 422-431 (1996)
Nuclear size is sensitive to NTF2 protein levels dependent on Ran binding
Vukovic LD, et al.
Journal of Cell Science, jcs-181263 (2016)
Separate binding sites on nuclear transport factor 2 (NTF2) for GDP-Ran and the phenylalanine-rich repeat regions of nucleoporins p62 and Nsp1p
Clarkson WD, et al.
Journal of Molecular Biology, 263(4), 422-431 (1996)
Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the ras-family GTPase ran1
Stewart M, et al.
Journal of Molecular Biology, 277(3), 635-646 (1998)
相关内容
Datasheet
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