N9527
Monoclonal Anti-NTF2 antibody produced in mouse
~2 mg/mL, clone 4F5, purified immunoglobulin, buffered aqueous solution
别名:
Anti-Nuclear Transfer Factor 2
生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
4F5, monoclonal
表单
buffered aqueous solution
分子量
antigen 14 kDa
种属反应性
human, hamster, monkey, mouse, rat, chicken, Xenopus
浓度
~2 mg/mL
技术
immunocytochemistry: suitable using 3.7% formaldehyde-0.2% Triton X-100 or 4% paraformaldehyde-methanol
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using HeLa cells cytosol preparation
同位素/亚型
IgG2b
UniProt登记号
运输
dry ice
储存温度
−20°C
基因信息
human ... NUTF2(10204)
mouse ... Ntf2(100043462)
rat ... Nutf2(291981)
一般描述
Nuclear transport factor 2 (NTF2 or NUTF2) is a cytosolic soluble protein. The polypeptide chain of NTF2 has α and β sheets and a hydrophobic cavity. NUTF2 is localized on the human chromosome on 16q22.1.
免疫原
recombinant human NTF2. The epitope lies within the extreme C-terminus of NTF2.
应用
Monoclonal Anti-NTF2 antibody produced in mouse has been used in western blotting.
生化/生理作用
Nuclear transport factor 2 (NTF2 or NUTF2) binds with Ras (Rat Sarcoma)-family GTPase (guanosine triphosphatase) Ran (Ras related nuclear protein) and facilitates the transport of Ran to the nucleus. NTF2 binds to nuclear pore complex (NPC) nucleoporin proteins with FxFG repeats especially p62 also binds preferably to GDP-Ran rather than GTP-Ran to form the complex GDP-Ran and p62 binds at different regions in the hydrophobic cavity of NTF2. The mutation in NTF2 hinders the binding and transport of Ran and formation the p62-NTF2-Ran complex.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
分析说明
The antibody may also be used for microinjection, in vitro nuclear protein import assays, and blocking of NTF2 binding to Ran.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
Fine mapping of 16q-linked autosomal dominant cerebellar ataxia type III in Japanese families
Hirano R, et al.
Neurogenetics, 5(4), 215-221 (2004)
Nuclear size is sensitive to NTF2 protein levels dependent on Ran binding
Vukovic LD, et al.
Journal of Cell Science, jcs-181263 (2016)
The 1.6 angstrom resolution crystal structure of nuclear transport factor 2 (NTF2)
Bullock TL, et al.
Journal of Molecular Biology, 260(3), 422-431 (1996)
Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the ras-family GTPase ran1
Stewart M, et al.
Journal of Molecular Biology, 277(3), 635-646 (1998)
Separate binding sites on nuclear transport factor 2 (NTF2) for GDP-Ran and the phenylalanine-rich repeat regions of nucleoporins p62 and Nsp1p
Clarkson WD, et al.
Journal of Molecular Biology, 263(4), 422-431 (1996)
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