N9539
Anti-Nedd9 (C-terminal region) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-CAS2, Anti-CASL, Anti-CASS2, Anti-CRK-associated substrate-related protein, Anti-HEF1
生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~120 kDa
种属反应性
human
包装
antibody small pack of 25 μL
浓度
~1.0 mg/mL
技术
indirect immunofluorescence: 1.5-3.0 μg/mL using human HS68 cells.120
western blot: 1-2 μg/mL using Jurkat cell lysates
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... NEDD9(4739)
mouse ... Nedd9(18003)
rat ... Nedd9(291044)
一般描述
NEDD9 (neural precursor cell expressed, developmentally down-regulated 9) is a non-catalytic multi-domain scaffolding protein. The gene contains retinoic acid response element (RARE) element in its promoter region, and is composed of seven exons. It encodes for a protein composed of 834 amino acids in humans, and contains an N-terminal SH3 (src homology 3) domain, followed by SD - substrate domain, SRR - serine rich region, FAT - focal adhesion targeting domain. Its C-terminal contains a DYDY motif (amino acids 628–631). This gene is expressed ubiquitously, and shows strong expression in lungs and lung cell lines. NEDD9 is a member of the Cas (Crk-associated substrate) family of scaffolding proteins.
NEDD9 gene is mapped to human chromosome 6p24.2.
应用
Anti-Nedd9 (C-terminal region) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.
生化/生理作用
Anti-Nedd9 (C-terminal region) specifically recognizes human Nedd9.
NEDD9 (neural precursor cell expressed, developmentally down-regulated 9) functions in the assembly of oncogenic kinase complexes such as focal adhesion kinase (FAK), ABL, SRC, Aurora-A (AURKA). It, therefore, controls signaling pathways implicated in processes such as cell cycle, migration, adhesion, invasion and chemotaxis that are involved in for tumorigenesis and metastases. In melanoma, glioblastoma, colon cancer and head and neck squamous carcinoma, the overexpression of this protein results in enhanced epithelial to mesenchymal transition, migration, and metastasis.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
制备说明
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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此项目有
Preclinical and clinical studies of the NEDD9 scaffold protein in cancer and other diseases.
Shagisultanova E et al
Gene, 567(1), 1-11 (2015)
TP53 transcription factor for the NEDD9/HEF1/Cas-L gene: potential targets in Non-Small Cell Lung Cancer treatment.
Rousseau B et al
Scientific Reports, 5, 10356-10356 (2015)
Timothy R Rebbeck
Seminars in radiation oncology, 27(1), 3-10 (2016-12-18)
Prostate cancer rates vary substantially by race, ethnicity, and geography. These disparities can be explained by variation in access to screening and treatment, variation in exposure to prostate cancer risk factors, and variation in the underlying biology of prostate carcinogenesis
Polina Y Kozyulina et al.
Molecular cancer research : MCR, 13(3), 423-438 (2014-10-17)
The dissemination of tumor cells relies on efficient cell adhesion and migration, which in turn depends upon endocytic trafficking of integrins. In the current work, it was found that depletion of the prometastatic protein, NEDD9, in breast cancer cells results
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