正在寻找类似产品? 访问 产品对比指南
一般描述
GenElute 细菌基因组DNA试剂盒可以简单、方便地从革兰氏阴性菌中分离高纯度基因组DNA。对于大多数革兰氏阳性菌，本试剂盒必须结合选购的溶菌酶 (L4919)来有效裂解较厚的肽聚糖细胞壁。为方便您制备溶菌酶储液，我们提供了一种稀释的革兰氏阳性溶解溶液。
该试剂盒将硅胶膜系统的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。
该试剂盒将硅胶膜系统的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。
应用
纯化所得的细菌基因组DNA可用于以下游应用：
- 限制性内切酶酶切
- PCR
- Southern印迹
- 克隆
The purified bacterial genomic DNA is ready for downstream applications such as:
GenElute™ Bacterial Genomic DNA Kits has been used:
- restriction endonuclease digestions
- PCR
- Southern blots
- cloning
GenElute™ Bacterial Genomic DNA Kits has been used:
- to isolate DNA
- to prepare DNA from E. coli strain
- to extract genomic DNA
生化/生理作用
细菌首先在含有离液盐的溶液之中裂解，以确保大分子彻底变性。之后加入乙醇，从而使DNA在溶解物通过微量离心管在硅胶膜上离心时，结合到膜上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。
预期的基因组DNA得率取决于细菌培养物的细胞密度、细菌种属以及所用的菌株。通过GenElute试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。
预期的基因组DNA得率取决于细菌培养物的细胞密度、细菌种属以及所用的菌株。通过GenElute试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。
特点和优势
- 典型DNA得率：15 μg - 20 μg
- 提供有适用于革兰氏阳性菌和革兰氏阴性菌的实验方案
- 可在2小时内获得优质基因组DNA
- 可纯化出A260/A280 比率介于1.6-1.9之间的DNA
- 起始材料：不超过1.5 mL培养物
- 预期得率：至多20 μg
- 洗脱体积：400 μl
- 所需时间：70 - 120分钟
- A260/A280比率：1.6 - 1.9
- 无需苯酚、氯仿或乙醇沉淀步骤
包装
Components included:
- Qty in kit:15 Part number: T5449 Component description: Collection Tubes, 2 mL
- Qty in kit:5 Part number: CP9471 Component description: GenElute Miniprep Binding Columns
- Qty in kit:2 Part number: P2308 Component description: Proteinase K
- Qty in kit:1 Part number: W0263 Component description: Wash Solution 1
- Qty in kit:1 Part number: B6553 Component description: Wash Solution Concentrate
- Qty in kit:1 Part number: B6678 Component description: Lysis Solution T
- Qty in kit:1 Part number: B8803 Component description: Lysis Solution C
- Qty in kit:1 Part number: C2112 Component description: Column Preparation Solution
- Qty in kit:1 Part number: R6148 Component description: RNase A solution
- Qty in kit:1 Part number: B6803 Component description: Elution Solution (10 mM Tris-HCl, 0.5 mM EDTA, pH 9.0)
- Qty in kit:1 Part number: L7539 Component description: Gram-Positive Lysis Solution
其他说明
更多信息，请见www.sigma-aldrich.com/genomicdna。
法律信息
GenElute is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Central nervous system, Respiratory system
储存分类代码
3 - Flammable liquids
闪点(°F)
77.0 °F - closed cup
闪点(°C)
25 °C - closed cup
法规信息
低风险生物材料
危险化学品
常规特殊物品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Hydrogen and butanol production from native wheat straw by synthetic microbial consortia integrated by species of Enterococcus and Clostridium
Valdez-Vazquez I, et al.
Fuel: The Science and Technology of Fuel and Energy, 159, 214-222 (2015)
Modulation of Global Transcriptional Regulatory Networks as a Strategy for Increasing Kanamycin Resistance of the Translational Elongation Factor-G Mutants in Escherichia coli
Mogre A, et al.
G3 (Bethesda, Md.), g3-300284 (2017)
Alexander W Eastman et al.
Genome announcements, 2(1) (2014-01-25)
Here we report the complete genome sequence of the bacterium Paenibacillus polymyxa CR1 (accession no. CP006941), which consists of one circular chromosome of 6,024,666 bp with 5,283 coding sequences (CDS), 87 tRNAs, and 12 rRNA operons. Data presented will allow for
Saliya Gurusinghe et al.
Molecules (Basel, Switzerland), 24(10) (2019-05-24)
Small molecule discovery has benefitted from the development of technologies that have aided in the culture and identification of soil microorganisms and the subsequent analysis of their respective metabolomes. We report herein on the use of both culture dependent and
Molecular characterization of serogroup 19 Streptococcus pneumoniae in the Czech Republic in the post-vaccine era
VZemlivckova H, et al.
Journal of Medical Microbiology, 67(7), 1003-1003 (2018)
实验方案
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
的GenElute™细菌基因组DNA试剂盒提供了一种从细菌中分离纯基因组DNA的简便方法。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持